Next Generation Sequencing Core (NGSU) (Charles R. Drew University)

The Next Generation Sequencing Core (NGSU) at the Charles R. Drew University’s Institute for Advanced Biomedical Research (IABR) houses an Illumina NextSeq 2000 and an Ion Torrent S5 prime sequencer along with an automated library preparation solution using the Ion Chef system. We can perform RNA sequencing, and exome sequencing. Other services include RNA, DNA quality assessment (Agilent Tape Station or Bioanalyzer), library preparation, library quantitation (qPCR, Qubit) and sequencing. We also provide basic data interpretation service via the CLC genomics software and Ingenuity pathway analysis (IPA) for RNA and Exome sequencing. Variant calling is available via the NextSeq 2000 Dragen germline or somatic pipelines. Other open-source data analysis approaches for RNA sequencing results (for e.g STAR-RSEM, Kallisto, Salmon) are also provided. The Ion Torrent platform can run targeted variant discovery or RNA expression. It currently uses the Ion Torrent Reporter system for data analysis. 

For more information, please contact: Sequencing@cdrewu.edu. 

The IABR also provides flow cytometry (Attune Nxt and BD FACS Melody) and microscopy services (LEICA SPE 3-color confocal, Cytation 1 live imaging system). 

For further information about the services and equipment available in IABR, please visit the Institute’s website (IABR@cdrewu.edu) or contact Pranab Dutta, PhD (pranabandutta@cdrewu.edu).

Last updated
March 19, 2025