Research Cores

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The Translational Research Core provides pathology and molecular services which include grossing of animal tissue, histopathology, tissue microarray generation, and the development of protocols for new antibodies and in-situ hybridization probes. Imaging and image analysis services include: (1) full slide scanning of human and animal cells/tissues on glass slides, including either chromogenic or fluorescent multispectral scanning, (2) image analysis of digital images generated with single or multiple probes in visible light or fluorescent wavelength ranges. The core also has a number of state-of-the art imaging and molecular instrumentation (laser-capture microdissection, nanostring, confocal, lightsheet microscope etc.) that are available to researchers.

Website:

https://www.cedars-sinai.org/research/cores/biobank-translational.html

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The Biobehavioral Research Core offers behavioral testing in rodents that ranges from the assessment of general health and neurological function (e.g., sensory and motor function) to the measurement of complex behaviors such as social interaction, memory, and learning. Assessments can be utilized for: (1) standard neurobehavioral assessment, (2) behavorial phenotyping, and (3) undesirable behavorial effects of drugs and biologics (gene, vaccine and stem cell therapies).

Website:

https://www.cedars-sinai.edu/Research/Research-Cores/Biobehavioral-Research-Core/

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The Mitochondria, Metabolism, and Mouse Cardiac Phenotyping Core provides services for isolation of mitochondria from a range of tissues for respirometry and other mitochondrial studies. The Core also provides cardiac ultrasound for Ejection Fraction and Fractional Shortening, and surgical models of heart disease including transverse aortic constriction (thoracic or abdominal), permanent or reversible coronary artery ligation, or pulmonary artery banding.

Website:

https://www.cedars-sinai.org/research/cores/metabolism-mitochondrial.html

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The Cedars-Sinai Research Imaging Core is an advanced imaging center dedicated to providing high-quality imaging services tailored to the unique needs of the research community. We offer a Clinical 3T MRI (Vida, Siemens) and simultaneous Clinical PET/MR (Biograph, Siemens) for human and large animal subjects, as well as 9.4T Small Animal MRI (Bruker), in-Vivo Small Animal/Specimen CT (Scanco 40), Optical (IVIS Spectrum and Luminar XR), Small Animal image guided irradiator (X-RAD SmART) and Small Animal 4Dx Lung imaging services for research. Our facility is staffed with highly trained and experienced personnel, including expert research scientists, technologists, registered nurses, a veterinarian, and additional support staff to ensure the most positive experience. We also offer core IRB/IACUC protocol support and seed grants to help get your research started quickly, and a dedicated research PACS and post-processing software to support data transfer, image analysis and data processing. In addition to research imaging, the Siemens systems are licensed for clinical use to allow standard-of-care, diagnostic imaging performed in conjunction with research imaging. We welcome and look forward to working with all CTSI investigators.

Website:

https://www.cedars-sinai.org/research/cores/imaging.html

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The Rodent Genetics Core provides specialized services for the production of genetically modified animals. The core offers high-quality, specific pathogen-free transgenic and knockout (knock-in) mice focused on genes of specific interest to a broad range of biomedical research projects to investigators at Cedars-Sinai, as well as affiliated and outside institutions. The core also helps design the DNA constructs for pronuclear microinjection or gene targeting with the most up-to-date genetic technologies such as the CRISPR/Cas9 genome editing system. The core also offers assisted fertilization services followed by embryo transfer to save mutant mouse lines, as well as cryopreservation of embryos and sperm for conservation of genetic resources. The core encourages interactive usage of these animals among Cedars-Sinai or CTSI affiliated investigators.

Website:

https://www.cedars-sinai.org/research/cores/rodent-genetics.html

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The Lundquist/Harbor-UCLA Animal Care and Use Program is uniquely designed to accommodate large animal models, and is equipped with several large animal surgical suites containing anesthesia machines, monitoring equipment, fluoroscopes, and a CT Scanner. The major types of studies center around perinatal lung development, the effect of the fetal environment on development, antibody production, vascular implant surgery, and cardiopulmonary resuscitation. Services include: (1) protocol development, (2) animal procurement, (3) animal care procedures, (4) animal health programs, (5) special services, and (6) training programs.

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Expertise and equipment for the design, implementation, and analysis of behavioral experiments. We work on a broad range of projects in both rats and mice, from behavioral phenotyping of novel transgenic mouse lines to developing rodent models of human clinical disorders.

Website:

http://btc.psych.ucla.edu/

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The overall goal of the Center for Aids Research (CFAR) Humanized Mouse Core is to provide the infrastructure, materials, animals, technical expertise, and support that will facilitate the use of humanized immunodeficient mice in studies examining the pathogenesis and treatment of HIV-induced disease and cancer. Services include: (1) procuring immunodeficient mice at a reduced cost, (2) providing specialized animal laboratories for the construction, housing, and maintenance of immunodeficient mice, (3) constructing humanized mice, (4) developing and optimizing new humanized mouse models, (5) providing technical expertise and consultation on humanized mouse models. The core also provides consultation in flow cytometry, immunohistochemistry, PCR, and other assays.

Website:

https://www.uclahealth.org/aidsinstitute/cfar/humanized-mouse-core

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The ICP-MS Core Facility provides analytical chemistry characterization services to determine the qualitative and quantitative composition with wide element coverage, up to 80 elements. Chemical analysis is completed at a part per trillion (ppt, pg/mL) scale with high sensitivity. Trained staff will provide core services including: (1) Consultation for effective decision-making based on your needs, (2) Sample preparation (e.g., acid digestion), (3) Conduct analyses, and (4) Data reports with background corrections. Applications for the Core services include (but not limited to): (a) Drug delivery (drug release kinetics, biodistribution in different organs), (b) Concentration measurements of metal-containing proteins, (c) Cellular uptake, (d) Impurities / composition / stoichiometric ratio / ion release kinetics, (e) Toxic elements analysis (As, Pb, Cd, Hg, etc.) in food and soil, and (d) Trace metal analysis in drinking water and wastewater.

Website:

http://www.cein.ucla.edu/new/p193.php?pageID=409

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The Mouse Physiology Core Laboratory facility is dedicated to the phenotypic assessment of mouse and rat models of disease using a wide variety of integrative approaches. Our aim is to provide a cost effective means for investigators to reveal important functional aspects of their mouse and rat models without having to individually invest in hiring skilled personnel and in the purchase of expensive instrumentation. We specialize in ultrasound (echo) & telemetric assessments, survival and non-survival surgeries, acute and chronic evaluations of cardiac and neuro-regulatory function as well as basic metabolic and exercise assessments. The lab is also capable of certification testing of new drugs or substances intended for human use by performing FDA approved (GLP certified 21 CFR part 58 including part 11 compliance) studies on rodent models.

Website:

http://www.physiology.ucla.edu/Labs/Mouselab.htm

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The Radiation Oncology Animal Core breeds and houses mice in a gnotobiotic environment. The core can supply various strains of mice to meet investigators’ needs (please see website). Mice from other facilities may be imported, after inspection of sentinel health reports, and housed in the extra-barrier area. Irradiators are available for both whole-body and partial body exposures including image-guided techniques. The core additionally maintains an ABSL2 facility.

Website:

https://www.uclahealth.org/radonc/radiation-oncology-animal-core

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The Zebrafish Core facilitates the use and quick access of common mutation, genetically-engineered transgenic zebrafish, and routine techniques of zebrafish manipulation by investigators. Services include: (1) providing space to house zebrafish and perform large-scale genetic screens, (2) providing embryos for experiments, (3) generation of transgenic zebrafish, and (4) cryostorage of zebrafish sperm and re-derivation of live fish. Training is available for select techniques and zebrafish husbandry.

Website:

https://www.mcdb.ucla.edu/Research/Zebrafish/

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The Biobank is a state-of-the-art biorepository that: (1) collects human biospecimens based on institutional priorities for biomedical research and also for investigator-driven requests, (2) provides tissue, blood, and body fluid collection, processing, and storage services, (3) provides a uniform, institution-wide, consent process for facilitating research studies, (4) provides clinical annotation services and fulfills custom requests for chart review, and (5) aids in developing protocols for human biospecimen procurement and processing.

The Translational Research Core provides pathology and molecular services which include grossing of human/animal tissue, histopathology, tissue microarray generation, and the development of protocols for new antibodies and in-situ hybridization probes. Imaging and image analysis services include: (1) full slide scanning of human and animal cells/tissues on glass slides, including either chromogenic or fluorescent multispectral scanning, (2) image analysis of digital images generated with single or multiple probes in visible light or fluorescent wavelength ranges. The core also has a number of state-of-the art imaging and molecular instrumentation (laser-capture microdissection, nanostring, confocal, lightsheet microscope etc.) that are available to researchers.

Website:

https://www.cedars-sinai.org/research/cores/biobank-translational.html

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The CSMC Flow Cytometry Core is equipped with state-of-the-art equipment for cell analysis and sorting, utilizing fluorescent probes targeted to specific cell-associated molecules to characterize the diversity and function of complex cell populations. Fluorescence-activated cell sorters can also individually identify and isolate live cells with a defined phenotype that can later be expanded and/or further studied. The core is equipped to handle biohazardous samples and offers both, cell population and single cell sorting. The core is open 24/7 for analysis and can provide flexible hours for sorting to accommodate experimental needs. The core also provides training for new users and offers consulting for experimental design, for a fee.

Website:

https://www.cedars-sinai.org/research/cores/flow-cytometry.html

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The HLA and Immunogenetics Laboratory provides state-of-the-art histocompatibility and immunogenetics testing for solid organ and hematopoietic stem cell transplantation. The HLA Laboratory also provides histocompatibility testing for non-transplant services including disease association and drug hypersensitivity. Services include: (1) intermediate and high-resolution HLA typing by next generation sequencing, (2) HLA and non-HLA antibody detection by solid phase or cell-based assays, (3) KIR and MICA gene typing, (4) chimerism testing, and (5) other immune assessment.

Website:

https://www.cedars-sinai.org/programs/transplant-center/programs/immunogenetics.html

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The Induced Pluripotent Stem Cell (iPSC) Core at the Regenerative Medicine Institute uses the latest techniques to reprogram, expand, and characterize human iPSCs from human skin or blood tissues of healthy subjects and diseased patients. iPSCs are then turned into specific cells of the human body including components of the nervous system, eyes, blood, bones, heart, gut, liver, and pancreas, for use by researchers. Applications of this technology include human disease modeling-in-a-dish, developing human reporter cell lines via genetic modification, drug screening on pathological human cell types, and potentially developing cell replacement or regenerative therapies. The iPSC Core offers: (1) PSC quality control and characterization services, (2) bio-banking and PSC thawing, (3) imaging services, (4) cell lines, (5) custom MEF generation, and (6) training.

Website:

https://www.cedars-sinai.org/research/areas/biomanufacturing.html

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The Mitochondria, Metabolism and Cardiac Phenotyping Core provides comprehensive services for analysis of metabolism and mitochondrial function in the research setting, with plans to expand to provide clinical services. For in-depth mitochondrial characterization, services may include seahorse respirometry, seahorse XFp, mitochondrial isolation from tissue, seahorse data analysis, keyence microscope, indirect calorimetry (CLAMS), CLAMS data analysis, body composition (NMR), citrate synthase, complex 1, Amplex Red, Swelling Assay, and mtDNA/nucDNA. In-depth mitochondrial characterization may include mitochondrial morphometry, turnover rates, respirometry, membrane potential, mPTP susceptibility, cytochrome c release, free radical production, mtDNA sequencing and 8- oxo-dG analysis.

Website:

https://www.cedars-sinai.org/research/cores/metabolism-mitochondrial.html

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The Bioimaging and Immunotherapeutics Research Core provides four major resources to researchers: (1) the BD Calibur flow cytometer facility, which employs a dual-laser (argon and helium-neon), 4-color FACS Calibur flow cytometer and cell sorter to facilitate the molecular and physiological examination of individual cells and enable collection of living cells for further study; (2) the IVIS Lumina II system, a high-sensitivity, in vivo imaging technology platform that enables non-invasive visualization of both bioluminescent and fluorescent signals, tracking of cellular and therapeutic activities within a living organism in real time, and detection of signals from tissue, petri dishes, and microtiter plates; (3) a Luminex multiplex analyzer that utilizes xMAP microsphere technology and has the capability of performing up to 100 assays simultaneously in a single well of a microtiter plate; and (4) a Biotek Synergy 2 multi-mode microplate reader, a combination luminometer-fluorometer-absorbance detector capable of reading 96-well and larger plates that utilizes both filter-based and monochromatic-based light-detection technology.

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The High-Resolution Tissue Respirometry (HRTR) Core supports investigators in mitochondrial physiology and pathophysiology studies. The core provides consultation, training, and services for assessment of oxidative phosphorylation in isolated mitochondria, permeabilized cells, or tissue preparations. The HRTR Core uses Oroboros Oxygraph-2k High Resolution Respirometry equipment. Applications of HRTR include: (1) diagnosis of acquired and genetic mitochondrial diseases, myopathies, and neuromuscular pathologies, (2) study of pathologies with reduced cellular respiration, (3) studies on cell function and cell death, (4) aging and senescence, (5) oxidative stress and antioxidant systems, (6) ischemia-reperfusion injury, (7) cancer research / pharmacological tests, (8) environmental stress, and (9) metabolic substrate balance.

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The Cellular Bioenergetics Core provides access to the XF24-3, a unique instrument that quantifies the activity of two major cellular energetic pathways, mitochondrial respiration and glycolysis, in cultured cells. The XF Technology: (1) measures O2 consumption rate, extracellular acidification rate, and CO2 production rate of living cells simultaneously in real time, (2) does not require the addition of dyes, labels, or reporters, (3) is capable of assaying cell lines, primary cells, isolated mitochondria, and small tissue pieces, (4) typically requires only 30,000 to 80,000 cells/well in 24-well plates, (5) measures adherent cells without requiring trypsinization, (6) does not affect cells and plates, which can then be used for an additional assay, (7) allows measurements to be repeated multiple times to measure kinetic responses, and (8) accommodates the addition of up to four test compounds (drugs or substrates) to each well, with measurements performed before and after the addition of each compound.

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The Center for Aids Research (CFAR) Virology Core Laboratory provides a wide array of services for both HIV/AIDS and other researchers. It offers testing services (p24 ELISA, real time and digital droplet PCR for the detection of HIV cDNA, HIV viral load), blood and blood cell products. In addition, the core maintains a fully equipped and stocked BSL2+ facility for scientists that do not have wet lab space, new investigators joining UCLA or investigators that do not have such lab space. The UCLA/CFAR Virology Core Laboratory also maintains real time PCR instrumentation and a QX200 Bio Rad Digital Droplet PCR System. Finally,the core is able to assist individual researchers with special projects related to, but not limited, HIV detection.

Website:

http://www.virology.med.ucla.edu/

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The core laboratory provides instrumentation and technical and professional assistance for performing laser-based flow cytometric analysis and cell sorting. The facility also offers training in basic flow cytometry principles and in the operation of analytic cytometers. A second course teaches nucleic acid analysis and measurement of proliferation by flow cytometry as well as hands-on instrument set-up and use of the specialized DNA analysis software.

Website:

http://cyto.mednet.ucla.edu/home.html

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The Immune Assessment Core (IAC) is a CLIA certified laboratory that provides comprehensive immunological testing services for basic, clinical, and translational studies. The IAC provides both standardized and customized multi-parameter flow cytometry, multiplexed immunoassays and cellular immune function assays to evaluate the innate and adaptive immune status of study subjects. Validated tests include comprehensive immunophenotyping panels, multiplexed cytokine/chemokine detection in body fluids or culture supernatants, several ELISA assays, allo- and virus-specific T cell activation, T and B cell proliferation to antigens and mitogens, NK cell cytotoxicity, and granulocyte/monocyte oxidative burst. The core also performs customized assay development to meet the investigators’ needs.

Website:

http://pathology.ucla.edu/iac

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The Immuno/BioSpot Core specializes in assays related to the detection, quantification, and qualification of biological spot assays and single-cell related analyses, including ELISPOT, colony counting, plaque assays, FluoroSpot, cell viability tests, apoptosis tests, in vivo/in vitro cytotoxicity measurements using cells labeled with fluorescent dyes, histochemistry stains, genotoxicity assays, multi-color intracellular/surface quantification, etc.

Website:

Immuno/BioSpot Core

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The ICP-MS Core Facility provides analytical chemistry characterization services to determine the qualitative and quantitative composition with wide element coverage, up to 80 elements. Chemical analysis is completed at a part per trillion (ppt, pg/mL) scale with high sensitivity. Trained staff will provide core services including: (1) Consultation for effective decision-making based on your needs, (2) Sample preparation (e.g., acid digestion), (3) Conduct analyses, and (4) Data reports with background corrections. Applications for the Core services include (but not limited to): (a) Drug delivery (drug release kinetics, biodistribution in different organs), (b) Concentration measurements of metal-containing proteins, (c) Cellular uptake, (d) Impurities / composition / stoichiometric ratio / ion release kinetics, (e) Toxic elements analysis (As, Pb, Cd, Hg, etc.) in food and soil, and (d) Trace metal analysis in drinking water and wastewater.

Website:

http://www.cein.ucla.edu/new/p193.php?pageID=409

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The IMTC services include (but are not limited to): (1) consultation and troubleshooting in techniques for efficient gene delivery, as well as cloning and functional protein expression in mammalian cells in vitro and in vivo, (2) consultation and to perform customized gene expression profiling, cytological phenotype profiling, and high-throughput molecular screening studies by utilizing high-throughput and highly automated robotic systems. The Integrated Molecular Technologies Core (IMTC) is therefore intended to serve as a comprehensive resource that will provide access to state-of-the-art molecular tools and thereby aims to facilitate the research activities of CURE: DDRCC investigators.

Website:

https://www.ucladdrcc.med.ucla.edu/cores/integrated-molecular-technologies-core-imtc-and-microbiome-sub-core

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The UCLA Mitochondrial and Metabolism Core provides the necessary technical and conceptual resources for studying mitochondrial function. Services include respirometry assays in intact cells, permeabilized cells, and isolated mitochondria using the Seahorse XF96 Extracellular Flux Analyzer. An Operetta High Content Imaging System is also available for measurements of mitochondrial mass, membrane potential and reactive oxygen species, among other applications. The Mitochondrial and Metabolism Core provides in-depth training and scheduled use of instruments or full service options for assay design, performance, and data analysis.

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The Biostatistics & Bioinformatics Core is a resource for biostatistical and bioinformatics consulting and related methodological research, and serves as a focal point from which investigators may draw statistical expertise for planning, management, and analysis of their studies. Faculty and staff at the Biostatistics and Bioinformatics Core: (1) coordinate and manage statistical activities at Cedars-Sinai to ensure that investigators have ready access to statistical consultation and support, (2) provide statistical expertise in the design of experiments and studies, including research proposal development, sample size determination, randomization procedures and plans for interim reviews and final analysis, (3) assist with the writing of statistical components of manuscripts, (4) review the integrity and statistical soundness of all studies involving human subjects, (5) provide statistical analysis for projects using appropriate statistical and computing methodologies and assist in the interpretation and presentation of results, (6) interact and collaborate with the Clinical Research Office in the development of protocols and the monitoring and reporting of clinical data, (7) maintain a computing facility with up-to-date software for statistical analysis to support program project investigators, and (8) conduct biostatistical and bioinformatics methodology research on practical problems arising in basic science populational and clinical studies.

Website:

https://www.cedars-sinai.edu/Research/Research-Cores/Biostatistical-Sciences/

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The AI in Imaging and Neuroscience Core provides automated and semi-automated processing of medical images with a special focus on vascular diseases and cancer using advanced pattern recognition and machine learning algorithms. A web-platform that offers a cloud computing pipeline is provided for the application of artificial intelligence to medical images and physiological signals. The services are centered on the processing of magnetic resonance imaging (MRI), computed tomography (CT), digital subtraction angiography (DSA), Transcranial Doppler ultrasound (TCD), intracranial pressure waveforms (ICP), digital pathology slides and time lapsed live cell microscopy videos.

Website:

http://web.cs.ucla.edu/~fab/core.html

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The Computing Technologies Research Laboratory (CTRL) specializes in research and clinical data collection, management, and reporting solutions. They develop Internet-accessible, web-based database applications that enable and enhance clinical & basic research and education, in the biomedical community. Services include: (1) web application development, (2) mobile app programming, (3) database design and hosting, and (4) digital video production / telemedicine for conferencing and the classroom.

Website:

https://dgit.healthsciences.ucla.edu/pages/Software-Development

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The primary objective of DOMStat is to provide study design and statistical analysis collaboration to investigators in the Department of Medicine. DOMStat offers faculty and staff level statistical collaboration for grant proposals, data analysis, manuscript preparation, and other research activities requiring statistical input.

Website:

http://domstat.med.ucla.edu

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The Informatics Center for Neurogenetics and Neurogenomics (ICNN) provides CNN faculty experts in statistical genetics, gene expression analysis, and bioinformatics will oversee the activities of highly-trained staff members who will accomplish three goals: 1) provide expert consultation and analyses for neurogenetics and neurogenomics projects; 2) develop and maintain a shared computing resource that will include a computational cluster for computation-intensive analyses, web-servers and state-of-the-art software tools for a wide range of applications (including user-friendly versions of public databases, as well as workstations on which ICNN users will be trained to employ these tools; and 3) provide hands-on training in analysis and informatics to the users. ICNN is developing a whole-genome data analysis pipeline, aimed at filtering and prioritizing genetic variation identified in whole-genome sequencing studies for further study.

Website:

https://www.semel.ucla.edu/research/core/informatics-center-neurogenetics-and-neurogenomics-icnn

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The Semel Institute Biostatistics Core (SIStat) is a team of expert biostatisticians, methodologists, and research support staff. The mission of the core is to foster research productivity and quality by helping faculty design sound research projects, obtain independent funding, and correctly analyze data. Services include: (1) development of electronic data entry systems, (2) biostatistics services, (3) development of patient assessment software, and (4) development of administrative systems software. Support is offered for projects ranging from small pilot studies to large, long-running, multi-site center studies.

Website:

http://www.sistat.ucla.edu/

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The Applied Genomics, Computation & Translational Core (AGCT Core) is a fully equipped, automated, high-throughput genomics facility offering the latest in next generation and single cell sequencing technologies. Our goal is to generate and interpret single cell omics, genomics, transcriptomics, epigenomics, and metagenomics information for basic and clinical research. We offer general but complete laboratory and bioinformatics analysis pipelines to accommodate standard needs, as well as work actively in collaborations to offer more sophisticated or custom solutions on most omics-wide investigations.

As a 10x Genomic certified service provider, the AGCT Core specializes in single cell omics applications, including scRNA-Seq, scATAC-Seq, scV(D)J immune profiling, and scCNV. We also offer mRNA-Seq and total RNA-Seq for all sample types, including low-input or degraded RNA. Sequencing-only services and high-throughput qPCR are also available. The Core houses the latest in next-generation sequencing equipment, including Illumina’s NovaSeq, NextSeq, MiSeq, and MiniSeq, 10x Genomic’s Chromium Controller, automated liquid handlers, automated nucleic acid purification systems, nucleic acid fragment analyzers, and focused ultrasonicators.

The AGCT Core’s bioinformatics team provides analytical capabilities to support advanced biomedical research. We have set up solid pipelines associated with each sequencing service for primary data processing, including reads alignment, quality control and expression quantification (RNA-seq), variants calling (WGS/WES) and peak calling (ChIP-seq/ATAC-seq). We have also developed packages for in-depth, customized computational analyses driven by specific biological questions defined by investigators. Furthermore, we provide support on interpretation and presentation of results, generating extensive reports with publication-ready images and drafting of bioinformatics and statistical methodology for manuscripts. We also provide lifelong data curation and cloud storage for collaborators.

Please visit the AGCT Core’s website for a complete list of available services, instrumentation, and detailed information about data processing and analyses.

Website:

https://www.cedars-sinai.org/research/cores/genomics.html

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The Institute consists of 3 core laboratories—the Laboratory for Biochemistry, Molecular Phenotyping, and Microarray; the Laboratory for Statistical and Mathematical Genetics; and the Laboratory for Molecular Genetics, Bioinformatics, and High Throughput Genotyping. The Genomics Institute Faculty have been extremely active in large scale genetic studies and both single cohort and in multi-cohort (and multi-ethnic) consortium efforts that have identified many genetic loci for cardiometabolic traits. We have contributed to the design stages, to performance of biochemical phenotyping, to large scale genotyping, and to statistical genetic analysis. The many loci we and our collaborators have identified are being utilized by ourselves and others to generate multi-loci genetic risk scores as powerful tools to dissect disease etiology and to perform risk assessment in populations. Besides our involvement in many large scale genome wide association studies (GWAS), we are now actively involved in large scale whole genome sequencing studies (WGS) and Multi-Omics studies.

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The UCLA Microbiome Core performs services related to microbiome studies including human sample collection, fecal processing, 16S ribosomal RNA gene sequencing (including feces, tissues, intestinal lavage, rectal sponges, vaginal swabs), bioinformatics analysis of 16S rRNA data in relation to phenotypes/interventions, quantitative PCR of total bacterial load, fecal calprotectin measurement, and long-term storage of fecal aliquots as well as extracted microbial DNA. The Microbiome Core also performs shotgun metagenomics and metatranscriptomics on a custom basis.

Website:

https://www.uclahealth.org/gastro/jacobs-lab/core-services

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The UCLA Neurosciences Genomics Core (UNGC) is currently operating an Illumina BeadLab 1000 high throughput SNP genotyping system (iScan), a Sequenom MassArray Compact mass spec and and two Illumina HiSeq 2500 next generation sequencing instruments in the Gonda research facility on the UCLA campus. Services include: RNA/DNA sequencing (Non UCLA neuro labs please inquire about service availability). Custom snp genotyping using the high throughput Illumina iSelect assays and with the Sequenom iPlex assay. Genotyping using all currently available Illumina Infinium whole genome chips. Gene expression using Illumina human expression chips. Whole genome methylation using Illumina Meth-450 chips. Project costs can be highly dependent on scale, with significant discounts potentially applying to larger projects.? For project specific pricing please contact? Joe DeYoung, Facility Manager.

Website:

https://www.semel.ucla.edu/ungc

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Technology Center for Genomics and Bioinformatics (TCGB) is a CAP-accredited, fully automated, high-throughput genomic facility equipped with all major next generation sequencing and microarray platforms. The Core provides pre-experiment consultation and post-experiment support, including preparation of grant applications and publications, and strategic planning for additional research steps. The TCGB also provides educational training to faculty, staff, and students to raise awareness on new directions and major discoveries in the areas of genomics and bioinformatics. Services include: (1) next generation sequencing, (2) microarray analysis, and (3) quality control services for RNA, miRNA, and DNA samples including RNA/DNA extraction, cDNA synthesis for quantitative-PCR, and qRT- PCR.

Website:

http://pathology.ucla.edu/tcgb

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The Biobank is a state-of-the-art biorepository that: (1) collects human biospecimens based on institutional priorities for biomedical research and also for investigator-driven requests, (2) provides tissue, blood, and body fluid collection, processing, and storage services, (3) provides a uniform, institution-wide, consent process for facilitating research studies, (4) provides clinical annotation services and fulfills custom requests for chart review, and (5) aids in developing protocols for human biospecimen procurement and processing.

The Translational Research Core provides pathology and molecular services which include grossing of human/animal tissue, histopathology, tissue microarray generation, and the development of protocols for new antibodies and in-situ hybridization probes. Imaging and image analysis services include: (1) full slide scanning of human and animal cells/tissues on glass slides, including either chromogenic or fluorescent multispectral scanning, (2) image analysis of digital images generated with single or multiple probes in visible light or fluorescent wavelength ranges. The core also has a number of state-of-the art imaging and molecular instrumentation (laser-capture microdissection, nanostring, confocal, lightsheet microscope etc.) that are available to researchers.

Website:

https://www.cedars-sinai.org/research/cores/biobank-translational.html

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The HLA and Immunogenetics Laboratory provides state-of-the-art histocompatibility and immunogenetics testing for solid organ and hematopoietic stem cell transplantation. The HLA Laboratory also provides histocompatibility testing for non-transplant services including disease association and drug hypersensitivity. Services include: (1) intermediate and high-resolution HLA typing by next generation sequencing, (2) HLA and non-HLA antibody detection by solid phase or cell-based assays, (3) KIR and MICA gene typing, (4) chimerism testing, and (5) other immune assessment.

Website:

https://www.cedars-sinai.org/programs/transplant-center/programs/immunogenetics.html

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The Mitochondria, Metabolism, and Mouse Cardiac Phenotyping Core provides services for isolation of mitochondria from human tissues including skin fibroblasts, endomyocardial biopsies, platelets, or peripheral blood mononuclear cells for respirometry studies.

Website:

https://www.cedars-sinai.org/research/cores/metabolism-mitochondrial.html

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The Cedars-Sinai Research Imaging Core is an advanced imaging center dedicated to providing high-quality imaging services tailored to the unique needs of the research community. We offer a Clinical 3T MRI (Vida, Siemens) and simultaneous Clinical PET/MR (Biograph, Siemens) for human and large animal subjects, as well as 9.4T Small Animal MRI (Bruker), in-Vivo Small Animal/Specimen CT (Scanco 40), Optical (IVIS Spectrum and Luminar XR), Small Animal image guided irradiator (X-RAD SmART) and Small Animal 4Dx Lung imaging services for research. Our facility is staffed with highly trained and experienced personnel, including expert research scientists, technologists, registered nurses, a veterinarian, and additional support staff to ensure the most positive experience. We also offer core IRB/IACUC protocol support and seed grants to help get your research started quickly, and a dedicated research PACS and post-processing software to support data transfer, image analysis and data processing. In addition to research imaging, the Siemens systems are licensed for clinical use to allow standard-of-care, diagnostic imaging performed in conjunction with research imaging. We welcome and look forward to working with all CTSI investigators.

Website:

https://www.cedars-sinai.org/research/cores/imaging.html

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The CTRC Bionutrition Core at Lundquist/Harbor-UCLA provides services to outpatient, overnight / extended stay and community based studies. A metabolic kitchen is dedicated to the preparation of nutrient controlled and regular meals. The Bionutrition Core has a metabolic assessment room with DXA, indirect calorimetry, BIA and anthropometric tools that integrates body composition, bone mineral density, and energy expenditure testing. The Bionutrition Core also provides state-of-the-art nutrition computing facilities with programs for nutrient analysis, semi-quantitative food frequency analysis, and design and specification of research diets. The Bionutrition Core personnel are experienced with REDCAP and can facilitate the collection and tracking of nutrition data for research studies. Training in nutrition research methodology is available to research fellows and other students.

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The Computed Tomographic (CT) Reading Core specializes in cardiac, vascular, and body composition imaging using advanced CT workstations, advanced quality control methods, systems storage, and analytical methods for multiple investigations. It provides unique services in atherosclerosis imaging including plaque quantification, perfusion imaging, and diagnostic services. In addition, the CT Reading Core offers collaborations in the area of renal, hepatic, vascular, and pulmonary imaging, as well as body composition and endothelial function assessment.

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The Endocrine and Metabolic Research Laboratory supports clinical and translational investigators in many aspects of research through expertise in analytical testing and development of new methods. Over forty different procedures are offered through the Endocrine and Metabolic Research Laboratory utilizing a variety of techniques including radioimmunoassay, fluoroimmunoassay, fluorometry and LC-MS/MS. Additionally, semen analyses and special andrological testing are available in the Andrology Laboratory.

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The pulmonary function testing (PFT) cardiopulmonary exercise testing (CPET) core lab facility supports investigators in pulmonary function and cardiopulmonary exercise testing studies. PFT consists of an array of non-invasive tests of abnormality of lung mechanics and pulmonary gas exchange used to detect the presence of, and quantify the severity of, a range of lung diseases. CPET provides a whole-body assessment of the integrative exercise responses involving the pulmonary, cardiovascular, hematopoietic, neuropsychological, and skeletal muscle systems, which are not adequately reflected through the measurement of individual organ system function. The CPET Core Lab will provide consultation, training, and services for clinical research using PFT and CPET. The Core Lab is also highly-experienced in multicenter clinical trials using PFT and CPET measurements.

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The facility offers DNA extractions from whole blood and other tissue types, and storage of extracted DNA. BSPC uses the Autopure LS™ nucleic acid purification instrument from Gentra Systems for extracting DNA. This instrument provides a highly automated process with very high quality DNA, samples are quantitated using OD 260/280. Once DNA has been obtained from the provided sample, it can be returned immediately or stored in -20?C freezer for future use. We'll also aliquot DNA at the desired concentrations. DNA can be stored indefinitely within BSPC. All samples are logged in our database and are bar-coded with our sample number and customer's identification code. BSPC will soon initiate RNA extraction.

Website:

https://www.semel.ucla.edu/research/core/biological-samples-processing-core---bspc

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The UCLA Center for Computer Vision and Imaging Biomarkers (CVIB) Laboratory provides global clinical and research services, including: (1) standardized multi-center imaging protocol development and quality control, (2) image de-identification, transfer, banking, and distribution, (3) cutting-edge quantitative image feature extraction, analysis, and data management, and (4) full service clinical trial management with regulatory compliance. CVIB has an exclusive contract with MedQIA Imaging CRO (www.medqia.com) to provide FDA-compliant services for industry-funded clinical trials worldwide. CVIB also provides clinical quantitative imaging services within UCLA Healthcare and to overseas hospital departments.

Key attributes of the CVIB Core Laboratory are: (1) image quality, (2) accurate and reproducible quantitation, (3) rigorous science, (4) high throughput, (5) well-managed and on-time service, and (6) user friendly.

Website:

http://cvib.ucla.edu/

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The Center for Human Nutrition provides Dual Energy X-Ray Absorptiometry (DXA or DEXA) Body Composition Analysis. DXA body composition analysis is considered the "gold standard" for measurement of body fat including visceral fat estimation, and gives an accurate measure for assessing health. Specialized scans to assess Bone Mineral Density analysis are available, which can be utilized for assessing bone health and risk estimation of osteopenia and osteoporosis.

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The Exercise Physiology Research Laboratory (EPRL) promotes a prevention model of wellness, focused on forestalling disease progression through the enhancement of health and fitness practices. Recently, the lab was distinguished as part of the Exercise is Medicine® on-campus recognition program through the American College of Sports Medicine, together whose vision is to create a campus culture that embraces physical activity and exercise as a daily facet of life and views it as essential for long-term health and wellness. Health-fitness assessments include: (1) aerobic performance; (2) pulmonary function; (3) body composition; (4) muscle performance; (5) functional movement screen; (6) total energy expenditure; (7) sleep quality; (8) cardiovascular health risk & HRV; (9) posture; and (10) performance tracking.

Website:

https://exerciselab.med.ucla.edu/

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The Immune Assessment Core (IAC) is a CLIA certified laboratory that provides comprehensive immunological testing services for basic, clinical, and translational studies. The IAC provides both standardized and customized multi-parameter flow cytometry, multiplexed immunoassays and cellular immune function assays to evaluate the innate and adaptive immune status of study subjects. Validated tests include comprehensive immunophenotyping panels, multiplexed cytokine/chemokine detection in body fluids or culture supernatants, several ELISA assays, allo- and virus-specific T cell activation, T and B cell proliferation to antigens and mitogens, NK cell cytotoxicity, and granulocyte/monocyte oxidative burst. The core also performs customized assay development to meet the investigators’ needs.

Website:

http://pathology.ucla.edu/iac

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The Immunogenetics Center provides comprehensive testing for organ and tissue transplantation to physicians, patients, pharmaceutical companies, and other medical facilities. It is one of the leading laboratories in the world, providing state-of-the-art technology and methodology with a comprehensive quality assurance program to ensure accuracy in testing. Services include: (1) HLA-class I (A,B,C) and class II (DR, DQ, DP) gene polymorphism typing, (2) histocompatibility testing (HLA antibody testing, MICA and endothelial cell antibody testing and crossmatch testing), (3) KIR and MICA gene typing and (4) immune assessment.

Website:

http://pathology.ucla.edu/uic-hla

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Two previous research laboratories, the Clinical and Translational Research Laboratory (CTRL) and the Clinical Immunology Research Laboratory (CIRL) have been restructured into the Pathology Research Portal (PRP). This new addition to the Center for Pathology Research Services functions as the biospecimen liaison between researchers and clinical testing, and provides coordination for sample receiving, accessioning, processing, short term and long term storage, dispatching to multiple core facilities for testing, and result retrieving. PRP also provides shipping and temporary storage services, and can also provide customized services to meet various research needs.

Website:

http://pathology.ucla.edu/ctrl

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The Transcranial Magnetic Stimulation (TMS) Lab providesTMS treatment to patients with Major Depressive Disorder (MDD) and other conditions.

Website:

http://www.tmslosangeles.com/index.php

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The Translational Pathology Core Laboratory (TPCL) provides an array of pathology-related services in support of basic, translational, and clinical research. Expert consultative services are offered to investigators in pathology-related study design, tissue selection, microscopic interpretation, immunohistochemistry/in situ hybridization, laser capture microdissection, digital image analysis, and IRB-related tissue questions. Services include: (1) tissue procurement, storage, and provision, (2) histology-related services, (3) immunohistochemistry, (4) in situ hybridization, (5) digital imaging and image analysis, (6) laser capture microdissection, (7) pathology consultative services, and (8) advice on research protocols, safety issues, and IRB applications.

Website:

http://pathology.ucla.edu/tpcl

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Imaging and image analysis services include: (1) full slide scanning of human and animal cells/tissues on glass slides, including either chromogenic or fluorescent multispectral scanning, (2) image analysis of digital images generated with single or multiple probes in visible light or fluorescent wavelength ranges. The core also has a number of state-of-the art imaging and molecular instrumentation (laser-capture microdissection, nanostring, confocal, lightsheet microscope etc.) that are available to researchers.

Website:

https://www.cedars-sinai.org/research/cores/biobank-translational.html

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The Cedars-Sinai Research Imaging Core is an advanced imaging center dedicated to providing high-quality imaging services tailored to the unique needs of the research community. We offer a Clinical 3T MRI (Vida, Siemens) and simultaneous Clinical PET/MR (Biograph, Siemens) for human and large animal subjects, as well as 9.4T Small Animal MRI (Bruker), in-Vivo Small Animal/Specimen CT (Scanco 40), Optical (IVIS Spectrum and Luminar XR), Small Animal image guided irradiator (X-RAD SmART) and Small Animal 4Dx Lung imaging services for research. Our facility is staffed with highly trained and experienced personnel, including expert research scientists, technologists, registered nurses, a veterinarian, and additional support staff to ensure the most positive experience. We also offer core IRB/IACUC protocol support and seed grants to help get your research started quickly, and a dedicated research PACS and post-processing software to support data transfer, image analysis and data processing. In addition to research imaging, the Siemens systems are licensed for clinical use to allow standard-of-care, diagnostic imaging performed in conjunction with research imaging. We welcome and look forward to working with all CTSI investigators.

Website:

https://www.cedars-sinai.org/research/cores/imaging.html

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The Bioimaging and Immunotherapeutics Research Core provides four major resources to researchers: (1) the BD Calibur flow cytometer facility, which employs a dual-laser (argon and helium-neon), 4-color FACS Calibur flow cytometer and cell sorter to facilitate the molecular and physiological examination of individual cells and enable collection of living cells for further study; (2) the IVIS Lumina II system, a high-sensitivity, in vivo imaging technology platform that enables non-invasive visualization of both bioluminescent and fluorescent signals, tracking of cellular and therapeutic activities within a living organism in real time, and detection of signals from tissue, petri dishes, and microtiter plates; (3) a Luminex multiplex analyzer that utilizes xMAP microsphere technology and has the capability of performing up to 100 assays simultaneously in a single well of a microtiter plate; and (4) a Biotek Synergy 2 multi-mode microplate reader, a combination luminometer-fluorometer-absorbance detector capable of reading 96-well and larger plates that utilizes both filter-based and monochromatic-based light-detection technology.

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The mission of the Advanced Light Microscopy and Spectroscopy Laboratory (ALMS) is to provide consultation, services, and support for the application of novel microscopic and spectroscopic methods and advanced image analysis techniques for the study of macromolecules, cellular dynamics, and nano-scale characterization of bio-materials. The facilities provide a collection of high-level, customized biological fluorescence microscopes and small-animal imaging devices that provide the ability to study biological processes with high spatial and temporal resolution in whole organisms and in living cells down to the single molecule detection level with nanometer accuracy. Services include: (1) wide-field fluorescence imaging microscopy (on a limited basis), (2) confocal one-photon and two-photon laser scanning microscopy (point scanning and spinning disk), (3) fluorescence correlation spectroscopy, (4) fluorescence resonance energy transfer, (5) microscopic and macroscopic fluorescence lifetime imaging with time-correlated-single-photon-counting and near-infrared detection, (6) stimulated emission depletion laser-scanning microscopy, (7) microscopic and macroscopic (small animal) spectral unmixing, and (8) laser capture microdissection.

Website:

https://alms.cnsi.ucla.edu/

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The Ahmanson-Lovelace Brain Mapping Center is committed to facilitating the development and growth of the field of Brain Mapping, a sub-discipline of neuroscience which uses brain imaging to better understand the structure and function of the human brain in health and alterations associated with disease. It is comprised of resources located in the core facility, the Ahmanson-Lovelace Brain Mapping Center, as well as additional resources housed in the Reed Neurological Research Center and the Neuroscience Research Building. Services include: (1) imaging (MRI and PET), (2) NeuroModulation, (3) data analysis, (4) data center, and (5) the DIVE (Data Immersive Visualization Environment).

Website:

http://www.bmap.ucla.edu/services/

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The Biological Chemistry Imaging Facility (BCIF) provides resources for data acquisition and analysis for radioactive, fluorescent, and photographic samples as well as digital imaging and document production. BCIF provides round-the-clock access to a cluster of modern equipment such as Typhoons 9410 and 9400 Variable Mode Imagers, Storm 820 and 840 imagers, Laser Densitometer, Gel Documentation System, color printers, and high-resolution scanners. In addition, BCIF provides unlimited scientific data storage space on its secure storage arrays to all participating labs and access to a number of software packages for data analysis and digital data processing.

Website:

http://www.biolchem.ucla.edu/biological-chemistry-imaging-facility

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The Electron Imaging Center for Nanomachines (EICN) provides state-of-the-art electron microscopy instruments and assisted usage services. Highly experienced staff provides necessary training and can help users address complex electron imaging needs. EICN offers advanced electron imaging techniques for visualizing materials, nanomachines, and cellular structures at atomic or nanometer scales in 2D and 3D. In addition, our latest integrated iCorr technology enables a streamlined approach to correlative microscopy to perform both fluorescence light and electron microscopy for biological research. Services available: (1) instrument training, (2) self usage, (3) assisted usage, and (4) data processing.

Website:

http://www.eicn.ucla.edu/

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The Electron Microscopy Services Center houses a JEOL 100CX transmission electron microscope. A Reichert Ultracut ultramicrotome is also available for use by trained personnel. Services include: (1) fixation and embedding of specimens, thin sectioning, (2) use of the electron microscopes (with or without assistance), (3) gold labeling, (4) negative stain, and (5) light microscopy of plastic embedded materials. The facility offers advice on appropriate preparatory procedures and other technical matters. Training and assistance in the use of the electron microscope is also offered.

Website:

http://www.bri.ucla.edu/research/core-facilities

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The Microscopic Techniques Laboratory offers instructions in microscopic techniques and assistance in tissue specimen preparation for light microscopic observation. Histological procedures available include: (1) some immunocytochemistry staining, (2) special stains, (3) paraffin sectioning, (4) slide preparation for in situ hybridization, (5) cryostat sectioning, (6) plastic embedding and (6) sectioning. The laboratory also provides staining setups, a cryostat, microtomes, and a Nikon photomicroscope for use by trained personnel.

Website:

http://www.bri.ucla.edu/research/core-facilities

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The MSSR is a core facility open to all research labs on campus. The services provided include the use of high throughput screening (HTS) technology, a total of roughly 200,000 compounds in various libraries, siRNA sets of the druggable genome for mouse and human and a database of results from screens. Please use the links above to find out more about the MSSR. The MSSR is currently funded by the JCCC, the Department of Molecular and Medical Pharmacology, the Department of Microbiology, Immunology and Molecular Genetics, and the Dean’s office, David Geffen School of Medicine and the California NanoSystems Institute.

Website:

https://mssr.cnsi.ucla.edu/

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The Nano and Pico Characterization (NPC) Laboratory provides Provides both state-of-the-art microscopic techniques to visualize surfaces, adsorbates, nanostructures and devices at the atomic and molecular scale as well as a unique opportunity for researchers to gain insight into local properties under a wide range of experimental conditions.

Website:

https://npc.cnsi.ucla.edu/

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Provides state-of-the-art small animal imaging. It functions both as a shared preclinical imaging resource for UCLA researchers and as a hub for emerging imaging research and technology development. The same technologies and services are also available to the larger research community including other academic institutes and industry groups through contract work. The Imaging Center, operating through its sales-and-service, offers microPET, microCT, bioluminescence and fluorescence imaging modalities and complementary in vitro/ex vivo services including cell-based assays, biodistribution, digital autoradiography and dosimetry. Companion PET tracer radiochemistry and radiolabeling services are available in-house and is supported by on-campus cyclotron facilities. Technical and analytical support are available throughout the study process: initial consultation, experimental design and optimization, imaging protocols and techniques, post-acquisition data analysis and interpretation. Training and staff assistance The Imaging Center is part of the Crump Institute for Molecular Imaging and is supported by the expertise of its faculty members, world leaders in various imaging sciences.

Website:

https://imaging.crump.ucla.edu/

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The Translational Research Imaging Center (TRIC) at UCLA is a state-of-the- art, pre-clinical and human cadaver, diagnostic and interventional imaging center. With over 25 years of expertise, our team of physicians, scientists, fellows, technologists, and veterinary staff support pre-clinical studies and imaging procedures across the field of medicine. TRIC is dedicated to the development and testing of new medical devices, imaging technologies, drug therapies as well as novel treatments. Our dedicated staff include: Board-certified Interventional Radiologists, Board-certified Radiologists, MR Physicists, Veterinarians, Statisticians, and Experienced Research Assistants. The TRIC Lab imaging equipment and support systems include: Magnetic Resonance Imaging (MRI) – Siemens Magnetom 3T Prisma MRI whole-body system, X-Ray Angiography – Siemens Artis Zeego Angiogram Suite with robotic C arm with 3D rotational angiography and DynaCT capabilities, Computed Tomography (CT) – Siemens Somatom Definition 64 Dual Source scanner, X-Ray Angiography – Philips Allura Xper FD-10 Angiogram Suite with floor mounted C arm with 3D rotational angiography capabilities, iU22 Philips Ultrasound system for general imaging, PACS data management system, observation and recovery suites, multi-modality 3D-image post-processing, High-Definition video integration for Telepresence video conferencing.

Website:

https://www.uclahealth.org/radiology/pre-clinical-services

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The Molecular Therapeutics Core provides a cross-disciplinary resource for researchers engaged in structural biology, therapeutics development, and drug discovery research. Services include: (1) pilot recombinant protein production, (2) large-scale protein production, (3) biophysical characterization, (4) molecular modeling and drug design, and (5) x-ray crystallographic services. Initial consultations are available free-of-charge.

Website:

https://www.cedars-sinai.edu/Research/Research-Cores/Molecular-Therapeutics-Core/

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The Proteomics & Metabolomics Core is defined by technically diverse instrumentation with the most advanced technologies available to the fields of proteomics, metabolomics and quantitative targeted protein assays. These technologies are dedicated to the identification and quantitative analysis of analytes from all types of biological samples including whole blood, plasma, CSF, cells or tissues and include samples originating from cell culture, animal models or patient-derived research studies. Services include a wide variety of discovery proteomics assays, identification of co- and post-translational modifications (phosphorylation, acetylation, methylation citrullination and more), as well as the fields leading methodologies for protein dynamics and protein-protein interactions. A selection of targeted mass spectrometry-based assays is available for quantification of protein panels associated with important cellular pathways and functions. One example is the MitoPlex. The MitoPlex uses heavy peptides to deliver MRM quantitation of 37 enzymes involved in central carbon chain metabolism and can be paired with quantification of 219 central carbon chain small molecule metabolomics assay. This small molecule assay is one of the two metabolite specific assays available. The other targeted metabolomic assay covers a panel of lipid molecules and is specifically designed to quantify 1153 lipid species from plasma. In addition to many mass spectrometry-based assays available, the core is equipped with two of the field’s leading immunoassay platforms, (1) ultra-sensitive digital ELISA, SIMOA HD-1 Analyzer (2) high-performance electrochemiluminescence ELISA platform, MESO QuickPlex SQ 12. All Proteomics and Metabolomic Core services include data analysis and a data release consultation. Advanced bioinformatic services for biological pathway analyzes and multi-omic data integration are also available through the core bioinformatic services.

Website:

https://www.cedars-sinai.org/research/cores/proteomics-metabolomics.html

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The Biomedical Mass Spectrometry Facility (BMSF) supports clinical research projects in clinical nutrition and metabolic research. The BMSF specializes in experimental design using stable isotopes and analytical methods (13C carbon tracing) for physiological or metabolic investigations. It provides unique services in isotopomer distribution analysis of metabolites (tracer-based metabolomics) using GC/MS (or LC-TOF). In addition, the BMSF offers collaborations in the area of metabolite profiling and modeling of metabolic systems with tracers. Services provided include: (1) sample preparation, (2) quantitative and qualitative analysis by GC/MS, IRMS and LC/MS, (3) consultation, (4) collaboration in metabolite profiling and tracer-based metabolomics, and (5) training on approved projects.

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The Guenther Molecular Biology Core supports investigators performing morphological, biochemical, cell and molecular biological studies both at cellular and subcellular levels. Consultation and training services are provided for localization of genes, RNA transcripts and proteins in complex tissues as well subcellular distribution in tissues. The laboratory houses five types of state-of the art equipment: an Agilent Bioanalyzer, two Applied Biosystem StepOne Plus Sequence Detection Systems, one Applied Biosystems 7900HT 384 well real-time quantitative PCR system, one Leica Confocal SP8 platform, and a Palm Microbeam. The Guenther Core also has a facility for immunohistochemistry studies.

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The Institute consists of 3 core laboratories—the Laboratory for Biochemistry, Molecular Phenotyping, and Microarray; the Laboratory for Statistical and Mathematical Genetics; and the Laboratory for Molecular Genetics, Bioinformatics, and High Throughput Genotyping. The Genomics Institute Faculty have been extremely active in large scale genetic studies and both single cohort and in multi-cohort (and multi-ethnic) consortium efforts that have identified many genetic loci for cardiometabolic traits. We have contributed to the design stages, to performance of biochemical phenotyping, to large scale genotyping, and to statistical genetic analysis. The many loci we and our collaborators have identified are being utilized by ourselves and others to generate multi-loci genetic risk scores as powerful tools to dissect disease etiology and to perform risk assessment in populations. Besides our involvement in many large scale genome wide association studies (GWAS), we are now actively involved in large scale whole genome sequencing studies (WGS) and Multi-Omics studies.

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The IMTC services include (but are not limited to): (1) consultation and troubleshooting in techniques for efficient gene delivery, as well as cloning and functional protein expression in mammalian cells in vitro and in vivo, (2) consultation and to perform customized gene expression profiling, cytological phenotype profiling, and high-throughput molecular screening studies by utilizing high-throughput and highly automated robotic systems. The Integrated Molecular Technologies Core (IMTC) is therefore intended to serve as a comprehensive resource that will provide access to state-of-the-art molecular tools and thereby aims to facilitate the research activities of CURE: DDRCC investigators.

Website:

https://www.ucladdrcc.med.ucla.edu/cores/integrated-molecular-technologies-core-imtc-and-microbiome-sub-core

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The Integrated Nanomaterials Laboratory (INML) is a state-of-the-art nanomaterials synthesis and characterization facility. INML addresses the critical technological needs of the future through material development as nanoscience is integrated with disciplines such as electronics, photonics, renewable energy, chemistry, biology, and physics. Services include: (1) epitaxial services and research expertise and (2) molecular beam epitaxy facilities.

Website:

https://inml.cnsi.ucla.edu/

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The Integrated Systems Nanofabrication Cleanroom (ISNC) is a shared-use clean-room that provides state-of-the-art equipment, facilities, and technical expertise for fabricating the most demanding nano-scale structures and devices. The ISNC was created to support all aspects of nanofabrication-related research and to advance research and learning in nano-science. Services include: (1) e-beam lithography, (2) optical lithography, (3) thin-film deposition, (4) dry etch, (5) thermal processing, (6) metrology, and (7) access to integrated bio-suites.

Website:

https://isnc.cnsi.ucla.edu/

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Provides 3-dimensional structure of small molecules in solid crystals via X-ray crystallography. Equipped for characterization of polycrystalline solid materials using powder and thin film diffraction techniques. The laboratory is a full service facility where staff deals with all aspects of service from crystal selection to publication of results. In-house training is a requirement if you wish to use the equipment yourself. Part of the UCLA Molecular Instrumentation Center, which is a campus wide facility open to the UCLA community. The facility also collaborates with personnel from other universities and industry both locally and internationally.

Website:

http://uccore.org/ucla-j-d-mccullough-crystallography-laboratory-mic/

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The UCLA Lipidomics Laboratory provides the highest quality of quantitative lipid measurements from biological samples including cells, tissue, serum, and media. The lab offers automated sample preparation and two distinct mass spectrometry platforms: 1) shotgun lipidomics providing measurement of 1100 complex lipid species and 2) a fatty acid and sterols analysis compatible with isotopic labeling. Our full-service lab provides data analysis (including computational modeling of lipid flux parameters) and consultation services.

Website:

http://www.uclalipidomics.net/

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Consultation and technical assistance available to researchers prior to, and following, experiments. Evaluation of sample via Dynamic Light Scattering. Automatic setup of 4000 crystallization conditions per hour using 500uL of sample. (Hanging or Sitting Drops) Capable of distinguishing between organic and inorganic crystals using a sophisticated UV/vis microscope. Optimization of crystallization conditions. Storage of plates in temperature controlled incubators at 4?, 15?, and 20?C. Optimization of crystallization conditions using a library of 1,500 commercial compounds. Production of stereolithography files from Pymol, 3D design of labware, 3D printing.

Website:

http://www.doe-mbi.ucla.edu/crystallization/

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The Magnetic Resonance Facility houses six nuclear magnetic resonance (NMR) spectrometers and one electron paramagnetic resonance (EPR) spectrometer.

Website:

http://www.mic.ucla.edu/magnetic-resonance-spectroscopy

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The Mass Spectrometry Laboratory provides a wide range of sample characterization techniques for researchers. Mass spectrometry services include: (1) a variety of ionization methods including electron, chemical, matrix assisted laser desorption, direct analysis in real time, electrospray, and atmospheric pressure chemical ionization, (2) a variety of analyzers including time of flight, quadrupole, ion trap, Orbitrap, ion cyclotron resonance, and several tandem combinations of mass analyzers, and (3) microflow and nanoflow liquid chromatography. For proteomics studies, equipment is available for running and analyzing 1-D and 2-D gels, transferring mini- and mid-size gels, gel imaging, spot cutting, in-gel digestion, and protein/peptide identification. In addition, the facility provides consultation for sample preparation, experimental design, gel staining, analysis of protein expression patterns, western and lectin blots, in-gel trypic digestion, data mining, and operation of the various instruments. This laboratory is a part of the UCLA Molecular Instrumentation Center.

Website:

http://www.mic.ucla.edu/mass-spectrometry

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The Materials Characterization Laboratory offers thermal, optical, microscopic, electrical, and magnetic characterization of materials and elemental analysis of surfaces via a wide range of instruments including: (1) light scattering spectrometers, (2) several spectrophotometers, (3) scanning probe microscopes (AFM/STM), (4) a SQUID magnetometer, (5) a scanning electron microscope, and (6) an X-Ray photoelectron spectrometer. This laboratory is a part of the UCLA Molecular Instrumentation Center.

Website:

http://www.mic.ucla.edu/materials-characterization

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The UCLA Metabolomics Center enables investigators to study metabolism with a particular focus on central carbon metabolism (glycolysis, pentose phosphate pathway, TCA cycle, nucleos(t)ide synthesis, etc.). Metabolite analysis can be performed on a variety of specimens (e.g. cultured cells, culture medium, blood, urine, tissue). Generally, relative amounts of metabolites are measured, but absolute amounts can be determined as well. Tracing experiments using stable isotope-labeled metabolites (D, C-13, N-15) are routinely performed. For data analysis, relative amounts of metabolites as well as mass isotopologue distributions (if isotope tracing was performed) are calculated. Results are illustrated using bar graphs, heatmaps, principal component analysis, and mapping to Cytoscape maps.

Website:

http://systems.crump.ucla.edu/metabolomics_center/

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The MSSR is a core facility open to all research labs on campus. The services provided include the use of high throughput screening (HTS) technology, a total of roughly 200,000 compounds in various libraries, siRNA sets of the druggable genome for mouse and human and a database of results from screens. Please use the links above to find out more about the MSSR. The MSSR is currently funded by the JCCC, the Department of Molecular and Medical Pharmacology, the Department of Microbiology, Immunology and Molecular Genetics, and the Dean’s office, David Geffen School of Medicine and the California NanoSystems Institute.

Website:

https://mssr.cnsi.ucla.edu/

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The NMR Core Facility provides advanced NMR spectrometers and related equipment for the study of macromolecular structure and dynamics in solution by experienced users. In this capacity the core facility primarily provides the research tools in which to perform detailed measurements on complex biological systems. Non-expert members of the DOE laboratory receive access to NMR spectroscopy for more routine applications. These applications include: screening wild-type and mutated protein samples to determine their structural integrity, making small molecule binding affinity measurements, and temperature and denaturant studies of protein stability.

Website:

https://www.doe-mbi.ucla.edu/nuclear-magnetic-resonance-nmr-core-technology-center/

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Provides consultation, training, and access to and technical assistance for metabolomics, proteomics (top-down and bottom-up) and targeted small molecule quantitation using mass spectrometery and contemporary chromatography.

Website:

https://massspec.chem.ucla.edu/

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The Protein Expression Technology Center (PETC) was founded in 1994 to facilitate the expression and purification of proteins for structure/function studies. The PETC provides support in all aspects of protein expression from cloning through expression optimization and protein purification. The PETC is a UCLA-DOE Institute for Genomics and Proteomics facility but is open to all researchers.

Website:

http://www.doe-mbi.ucla.edu/expression/

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The UCLA Proteome Research Center is a full-service technology center that offers a comprehensive suite of proteomic services to the UCLA community and other partnering institutions. By bringing together state-of-the-art instrumentation and high- level technical staff, the goal of the facility is to transform traditional experimental paradigms by providing investigators access to powerful analytical workflows for systems-level characterization of complex biological systems, biomarker discovery and validation, and protein complex characterization. Available services include: (1) complex protein mixture analysis using single and multidimensional fractionation strategies, (2) peptide quantitation using SILAC, iTRAQ / TMT, and label-free methodologies, (3) targeted and global PTM enrichment and characterization approaches, (4) identification of protein interaction networks using affinity-based isolation and proximity tagging techniques, and (5) targeted proteomic assay development using parallel reaction monitoring (PRM) for relative and absolute quantitation.

Website:

http://www.uclaproteomics.net/

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The UCLA-DOE and Biochemistry Shared Instrumentation core facility provides the UCLA research community open access to sophisticated equipment for biochemical studies. These facilities house a wide array of detection systems useful for analysis, quantification, and identification of all types of macromolecules and their molecular interactions. It also carries equipment for cell culturing, harvesting, lysis, and high-speed centrifugation useful for biomolecule preparation.

Website:

http://biochem.cores.ucla.edu/general_info/

Last modified 02-07-2020

The X-ray Crystallography Core Technology Center in the Department of Engineering provides state-of-the-art resources to researchers, enabling the detailed 3-D analysis of biological macromolecules that play essential roles in human health. The facility operates as a full service core that offers access to sophisticated equipment and technologies as well as advice and technical assistance in sample preparation, data collection, processing, atomic refinement, and modeling. Services include: (1) aid in crystallization, (2) X-ray characterization of crystals, (3) X-ray data collection in-house and at the synchrotron, (4) processing and quality analysis of data, and (5) structure determination and display.

Website:

http://www.doe-mbi.ucla.edu/x-ray-crystallography-core-technology-center/

Last modified 02-07-2020

The Microelectronics Shop provides technical support for research, teaching, and administrative functions. Two IT Programmer/Analysts and one Electronics Engineer are on staff to provide electronic design and fabrication for custom electronics solutions to unique situations encountered in research projects. The staff has expertise in Macintosh, IBM clone computers, peripherals, printers, and network interfacing, including installation, setup, upgrades, demonstration, troubleshooting, repair, and modification services. The Microelectronics Shop also provides design, development, and installation of local area networks, specifying hubs, network cards, cable types, and software. Consultation is provided to determine appropriate hardware/software solutions.

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Last modified 02-07-2020

The Translational Research Core provides pathology and molecular services which include grossing of animal tissue, histopathology, tissue microarray generation, and the development of protocols for new antibodies and in-situ hybridization probes. Imaging and image analysis services include: (1) full slide scanning of human and animal cells/tissues on glass slides, including either chromogenic or fluorescent multispectral scanning, (2) image analysis of digital images generated with single or multiple probes in visible light or fluorescent wavelength ranges. The core also has a number of state-of-the art imaging and molecular instrumentation (laser-capture microdissection, nanostring, confocal, lightsheet microscope etc.) that are available to researchers.

Website:

https://www.cedars-sinai.org/research/cores/biobank-translational.html

Last modified 02-07-2020

The Biobehavioral Research Core offers behavioral testing in rodents that ranges from the assessment of general health and neurological function (e.g., sensory and motor function) to the measurement of complex behaviors such as social interaction, memory, and learning. Assessments can be utilized for: (1) standard neurobehavioral assessment, (2) behavorial phenotyping, and (3) undesirable behavorial effects of drugs and biologics (gene, vaccine and stem cell therapies).

Website:

https://www.cedars-sinai.edu/Research/Research-Cores/Biobehavioral-Research-Core/

Last modified 02-07-2020

The Mitochondria, Metabolism, and Mouse Cardiac Phenotyping Core provides services for isolation of mitochondria from a range of tissues for respirometry and other mitochondrial studies. The Core also provides cardiac ultrasound for Ejection Fraction and Fractional Shortening, and surgical models of heart disease including transverse aortic constriction (thoracic or abdominal), permanent or reversible coronary artery ligation, or pulmonary artery banding.

Website:

https://www.cedars-sinai.org/research/cores/metabolism-mitochondrial.html

Last modified 02-07-2020

The Cedars-Sinai Research Imaging Core is an advanced imaging center dedicated to providing high-quality imaging services tailored to the unique needs of the research community. We offer a Clinical 3T MRI (Vida, Siemens) and simultaneous Clinical PET/MR (Biograph, Siemens) for human and large animal subjects, as well as 9.4T Small Animal MRI (Bruker), in-Vivo Small Animal/Specimen CT (Scanco 40), Optical (IVIS Spectrum and Luminar XR), Small Animal image guided irradiator (X-RAD SmART) and Small Animal 4Dx Lung imaging services for research. Our facility is staffed with highly trained and experienced personnel, including expert research scientists, technologists, registered nurses, a veterinarian, and additional support staff to ensure the most positive experience. We also offer core IRB/IACUC protocol support and seed grants to help get your research started quickly, and a dedicated research PACS and post-processing software to support data transfer, image analysis and data processing. In addition to research imaging, the Siemens systems are licensed for clinical use to allow standard-of-care, diagnostic imaging performed in conjunction with research imaging. We welcome and look forward to working with all CTSI investigators.

Website:

https://www.cedars-sinai.org/research/cores/imaging.html

Last modified 02-07-2020

The Rodent Genetics Core provides specialized services for the production of genetically modified animals. The core offers high-quality, specific pathogen-free transgenic and knockout (knock-in) mice focused on genes of specific interest to a broad range of biomedical research projects to investigators at Cedars-Sinai, as well as affiliated and outside institutions. The core also helps design the DNA constructs for pronuclear microinjection or gene targeting with the most up-to-date genetic technologies such as the CRISPR/Cas9 genome editing system. The core also offers assisted fertilization services followed by embryo transfer to save mutant mouse lines, as well as cryopreservation of embryos and sperm for conservation of genetic resources. The core encourages interactive usage of these animals among Cedars-Sinai or CTSI affiliated investigators.

Website:

https://www.cedars-sinai.org/research/cores/rodent-genetics.html

Last modified 02-07-2020

The Biobank is a state-of-the-art biorepository that: (1) collects human biospecimens based on institutional priorities for biomedical research and also for investigator-driven requests, (2) provides tissue, blood, and body fluid collection, processing, and storage services, (3) provides a uniform, institution-wide, consent process for facilitating research studies, (4) provides clinical annotation services and fulfills custom requests for chart review, and (5) aids in developing protocols for human biospecimen procurement and processing.

The Translational Research Core provides pathology and molecular services which include grossing of human/animal tissue, histopathology, tissue microarray generation, and the development of protocols for new antibodies and in-situ hybridization probes. Imaging and image analysis services include: (1) full slide scanning of human and animal cells/tissues on glass slides, including either chromogenic or fluorescent multispectral scanning, (2) image analysis of digital images generated with single or multiple probes in visible light or fluorescent wavelength ranges. The core also has a number of state-of-the art imaging and molecular instrumentation (laser-capture microdissection, nanostring, confocal, lightsheet microscope etc.) that are available to researchers.

Website:

https://www.cedars-sinai.org/research/cores/biobank-translational.html

Last modified 02-07-2020

The CSMC Flow Cytometry Core is equipped with state-of-the-art equipment for cell analysis and sorting, utilizing fluorescent probes targeted to specific cell-associated molecules to characterize the diversity and function of complex cell populations. Fluorescence-activated cell sorters can also individually identify and isolate live cells with a defined phenotype that can later be expanded and/or further studied. The core is equipped to handle biohazardous samples and offers both, cell population and single cell sorting. The core is open 24/7 for analysis and can provide flexible hours for sorting to accommodate experimental needs. The core also provides training for new users and offers consulting for experimental design, for a fee.

Website:

https://www.cedars-sinai.org/research/cores/flow-cytometry.html

Last modified 02-07-2020

The HLA and Immunogenetics Laboratory provides state-of-the-art histocompatibility and immunogenetics testing for solid organ and hematopoietic stem cell transplantation. The HLA Laboratory also provides histocompatibility testing for non-transplant services including disease association and drug hypersensitivity. Services include: (1) intermediate and high-resolution HLA typing by next generation sequencing, (2) HLA and non-HLA antibody detection by solid phase or cell-based assays, (3) KIR and MICA gene typing, (4) chimerism testing, and (5) other immune assessment.

Website:

https://www.cedars-sinai.org/programs/transplant-center/programs/immunogenetics.html

Last modified 02-07-2020

The Induced Pluripotent Stem Cell (iPSC) Core at the Regenerative Medicine Institute uses the latest techniques to reprogram, expand, and characterize human iPSCs from human skin or blood tissues of healthy subjects and diseased patients. iPSCs are then turned into specific cells of the human body including components of the nervous system, eyes, blood, bones, heart, gut, liver, and pancreas, for use by researchers. Applications of this technology include human disease modeling-in-a-dish, developing human reporter cell lines via genetic modification, drug screening on pathological human cell types, and potentially developing cell replacement or regenerative therapies. The iPSC Core offers: (1) PSC quality control and characterization services, (2) bio-banking and PSC thawing, (3) imaging services, (4) cell lines, (5) custom MEF generation, and (6) training.

Website:

https://www.cedars-sinai.org/research/areas/biomanufacturing.html

Last modified 02-07-2020

The Mitochondria, Metabolism and Cardiac Phenotyping Core provides comprehensive services for analysis of metabolism and mitochondrial function in the research setting, with plans to expand to provide clinical services. For in-depth mitochondrial characterization, services may include seahorse respirometry, seahorse XFp, mitochondrial isolation from tissue, seahorse data analysis, keyence microscope, indirect calorimetry (CLAMS), CLAMS data analysis, body composition (NMR), citrate synthase, complex 1, Amplex Red, Swelling Assay, and mtDNA/nucDNA. In-depth mitochondrial characterization may include mitochondrial morphometry, turnover rates, respirometry, membrane potential, mPTP susceptibility, cytochrome c release, free radical production, mtDNA sequencing and 8- oxo-dG analysis.

Website:

https://www.cedars-sinai.org/research/cores/metabolism-mitochondrial.html

Last modified 02-07-2020

The Biostatistics & Bioinformatics Core is a resource for biostatistical and bioinformatics consulting and related methodological research, and serves as a focal point from which investigators may draw statistical expertise for planning, management, and analysis of their studies. Faculty and staff at the Biostatistics and Bioinformatics Core: (1) coordinate and manage statistical activities at Cedars-Sinai to ensure that investigators have ready access to statistical consultation and support, (2) provide statistical expertise in the design of experiments and studies, including research proposal development, sample size determination, randomization procedures and plans for interim reviews and final analysis, (3) assist with the writing of statistical components of manuscripts, (4) review the integrity and statistical soundness of all studies involving human subjects, (5) provide statistical analysis for projects using appropriate statistical and computing methodologies and assist in the interpretation and presentation of results, (6) interact and collaborate with the Clinical Research Office in the development of protocols and the monitoring and reporting of clinical data, (7) maintain a computing facility with up-to-date software for statistical analysis to support program project investigators, and (8) conduct biostatistical and bioinformatics methodology research on practical problems arising in basic science populational and clinical studies.

Website:

https://www.cedars-sinai.edu/Research/Research-Cores/Biostatistical-Sciences/

Last modified 02-07-2020

The Applied Genomics, Computation & Translational Core (AGCT Core) is a fully equipped, automated, high-throughput genomics facility offering the latest in next generation and single cell sequencing technologies. Our goal is to generate and interpret single cell omics, genomics, transcriptomics, epigenomics, and metagenomics information for basic and clinical research. We offer general but complete laboratory and bioinformatics analysis pipelines to accommodate standard needs, as well as work actively in collaborations to offer more sophisticated or custom solutions on most omics-wide investigations.

As a 10x Genomic certified service provider, the AGCT Core specializes in single cell omics applications, including scRNA-Seq, scATAC-Seq, scV(D)J immune profiling, and scCNV. We also offer mRNA-Seq and total RNA-Seq for all sample types, including low-input or degraded RNA. Sequencing-only services and high-throughput qPCR are also available. The Core houses the latest in next-generation sequencing equipment, including Illumina’s NovaSeq, NextSeq, MiSeq, and MiniSeq, 10x Genomic’s Chromium Controller, automated liquid handlers, automated nucleic acid purification systems, nucleic acid fragment analyzers, and focused ultrasonicators.

The AGCT Core’s bioinformatics team provides analytical capabilities to support advanced biomedical research. We have set up solid pipelines associated with each sequencing service for primary data processing, including reads alignment, quality control and expression quantification (RNA-seq), variants calling (WGS/WES) and peak calling (ChIP-seq/ATAC-seq). We have also developed packages for in-depth, customized computational analyses driven by specific biological questions defined by investigators. Furthermore, we provide support on interpretation and presentation of results, generating extensive reports with publication-ready images and drafting of bioinformatics and statistical methodology for manuscripts. We also provide lifelong data curation and cloud storage for collaborators.

Please visit the AGCT Core’s website for a complete list of available services, instrumentation, and detailed information about data processing and analyses.

Website:

https://www.cedars-sinai.org/research/cores/genomics.html

Last modified 02-07-2020

The Biobank is a state-of-the-art biorepository that: (1) collects human biospecimens based on institutional priorities for biomedical research and also for investigator-driven requests, (2) provides tissue, blood, and body fluid collection, processing, and storage services, (3) provides a uniform, institution-wide, consent process for facilitating research studies, (4) provides clinical annotation services and fulfills custom requests for chart review, and (5) aids in developing protocols for human biospecimen procurement and processing.

The Translational Research Core provides pathology and molecular services which include grossing of human/animal tissue, histopathology, tissue microarray generation, and the development of protocols for new antibodies and in-situ hybridization probes. Imaging and image analysis services include: (1) full slide scanning of human and animal cells/tissues on glass slides, including either chromogenic or fluorescent multispectral scanning, (2) image analysis of digital images generated with single or multiple probes in visible light or fluorescent wavelength ranges. The core also has a number of state-of-the art imaging and molecular instrumentation (laser-capture microdissection, nanostring, confocal, lightsheet microscope etc.) that are available to researchers.

Website:

https://www.cedars-sinai.org/research/cores/biobank-translational.html

Last modified 02-07-2020

The HLA and Immunogenetics Laboratory provides state-of-the-art histocompatibility and immunogenetics testing for solid organ and hematopoietic stem cell transplantation. The HLA Laboratory also provides histocompatibility testing for non-transplant services including disease association and drug hypersensitivity. Services include: (1) intermediate and high-resolution HLA typing by next generation sequencing, (2) HLA and non-HLA antibody detection by solid phase or cell-based assays, (3) KIR and MICA gene typing, (4) chimerism testing, and (5) other immune assessment.

Website:

https://www.cedars-sinai.org/programs/transplant-center/programs/immunogenetics.html

Last modified 02-07-2020

The Mitochondria, Metabolism, and Mouse Cardiac Phenotyping Core provides services for isolation of mitochondria from human tissues including skin fibroblasts, endomyocardial biopsies, platelets, or peripheral blood mononuclear cells for respirometry studies.

Website:

https://www.cedars-sinai.org/research/cores/metabolism-mitochondrial.html

Last modified 02-07-2020

The Cedars-Sinai Research Imaging Core is an advanced imaging center dedicated to providing high-quality imaging services tailored to the unique needs of the research community. We offer a Clinical 3T MRI (Vida, Siemens) and simultaneous Clinical PET/MR (Biograph, Siemens) for human and large animal subjects, as well as 9.4T Small Animal MRI (Bruker), in-Vivo Small Animal/Specimen CT (Scanco 40), Optical (IVIS Spectrum and Luminar XR), Small Animal image guided irradiator (X-RAD SmART) and Small Animal 4Dx Lung imaging services for research. Our facility is staffed with highly trained and experienced personnel, including expert research scientists, technologists, registered nurses, a veterinarian, and additional support staff to ensure the most positive experience. We also offer core IRB/IACUC protocol support and seed grants to help get your research started quickly, and a dedicated research PACS and post-processing software to support data transfer, image analysis and data processing. In addition to research imaging, the Siemens systems are licensed for clinical use to allow standard-of-care, diagnostic imaging performed in conjunction with research imaging. We welcome and look forward to working with all CTSI investigators

Website:

https://www.cedars-sinai.org/research/cores/imaging.html

Last modified 02-07-2020

Imaging and image analysis services include: (1) full slide scanning of human and animal cells/tissues on glass slides, including either chromogenic or fluorescent multispectral scanning, (2) image analysis of digital images generated with single or multiple probes in visible light or fluorescent wavelength ranges. The core also has a number of state-of-the art imaging and molecular instrumentation (laser-capture microdissection, nanostring, confocal, lightsheet microscope etc.) that are available to researchers.

Website:

https://www.cedars-sinai.org/research/cores/biobank-translational.html

Last modified 02-07-2020

The Cedars-Sinai Research Imaging Core is an advanced imaging center dedicated to providing high-quality imaging services tailored to the unique needs of the research community. We offer a Clinical 3T MRI (Vida, Siemens) and simultaneous Clinical PET/MR (Biograph, Siemens) for human and large animal subjects, as well as 9.4T Small Animal MRI (Bruker), in-Vivo Small Animal/Specimen CT (Scanco 40), Optical (IVIS Spectrum and Luminar XR), Small Animal image guided irradiator (X-RAD SmART) and Small Animal 4Dx Lung imaging services for research. Our facility is staffed with highly trained and experienced personnel, including expert research scientists, technologists, registered nurses, a veterinarian, and additional support staff to ensure the most positive experience. We also offer core IRB/IACUC protocol support and seed grants to help get your research started quickly, and a dedicated research PACS and post-processing software to support data transfer, image analysis and data processing. In addition to research imaging, the Siemens systems are licensed for clinical use to allow standard-of-care, diagnostic imaging performed in conjunction with research imaging. We welcome and look forward to working with all CTSI investigators.

Website:

https://www.cedars-sinai.org/research/cores/imaging.html

Last modified 02-07-2020

The Molecular Therapeutics Core provides a cross-disciplinary resource for researchers engaged in structural biology, therapeutics development, and drug discovery research. Services include: (1) pilot recombinant protein production, (2) large-scale protein production, (3) biophysical characterization, (4) molecular modeling and drug design, and (5) x-ray crystallographic services. Initial consultations are available free-of-charge.

Website:

https://www.cedars-sinai.edu/Research/Research-Cores/Molecular-Therapeutics-Core/

Last modified 02-07-2020

The Proteomics & Metabolomics Core is defined by technically diverse instrumentation with the most advanced technologies available to the fields of proteomics, metabolomics and quantitative targeted protein assays. These technologies are dedicated to the identification and quantitative analysis of analytes from all types of biological samples including whole blood, plasma, CSF, cells or tissues and include samples originating from cell culture, animal models or patient-derived research studies. Services include a wide variety of discovery proteomics assays, identification of co- and post-translational modifications (phosphorylation, acetylation, methylation citrullination and more), as well as the fields leading methodologies for protein dynamics and protein-protein interactions. A selection of targeted mass spectrometry-based assays is available for quantification of protein panels associated with important cellular pathways and functions. One example is the MitoPlex. The MitoPlex uses heavy peptides to deliver MRM quantitation of 37 enzymes involved in central carbon chain metabolism and can be paired with quantification of 219 central carbon chain small molecule metabolomics assay. This small molecule assay is one of the two metabolite specific assays available. The other targeted metabolomic assay covers a panel of lipid molecules and is specifically designed to quantify 1153 lipid species from plasma. In addition to many mass spectrometry-based assays available, the core is equipped with two of the field’s leading immunoassay platforms, (1) ultra-sensitive digital ELISA, SIMOA HD-1 Analyzer (2) high-performance electrochemiluminescence ELISA platform, MESO QuickPlex SQ 12. All Proteomics and Metabolomic Core services include data analysis and a data release consultation. Advanced bioinformatic services for biological pathway analyzes and multi-omic data integration are also available through the core bioinformatic services.

Website:

https://www.cedars-sinai.org/research/cores/proteomics-metabolomics.html

Last modified 02-07-2020

The Lundquist/Harbor-UCLA Animal Care and Use Program is uniquely designed to accommodate large animal models, and is equipped with several large animal surgical suites containing anesthesia machines, monitoring equipment, fluoroscopes, and a CT Scanner. The major types of studies center around perinatal lung development, the effect of the fetal environment on development, antibody production, vascular implant surgery, and cardiopulmonary resuscitation. Services include: (1) protocol development, (2) animal procurement, (3) animal care procedures, (4) animal health programs, (5) special services, and (6) training programs.

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The Bioimaging and Immunotherapeutics Research Core provides four major resources to researchers: (1) the BD Calibur flow cytometer facility, which employs a dual-laser (argon and helium-neon), 4-color FACS Calibur flow cytometer and cell sorter to facilitate the molecular and physiological examination of individual cells and enable collection of living cells for further study; (2) the IVIS Lumina II system, a high-sensitivity, in vivo imaging technology platform that enables non-invasive visualization of both bioluminescent and fluorescent signals, tracking of cellular and therapeutic activities within a living organism in real time, and detection of signals from tissue, petri dishes, and microtiter plates; (3) a Luminex multiplex analyzer that utilizes xMAP microsphere technology and has the capability of performing up to 100 assays simultaneously in a single well of a microtiter plate; and (4) a Biotek Synergy 2 multi-mode microplate reader, a combination luminometer-fluorometer-absorbance detector capable of reading 96-well and larger plates that utilizes both filter-based and monochromatic-based light-detection technology.

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Last modified 02-07-2020

The High-Resolution Tissue Respirometry (HRTR) Core supports investigators in mitochondrial physiology and pathophysiology studies. The core provides consultation, training, and services for assessment of oxidative phosphorylation in isolated mitochondria, permeabilized cells, or tissue preparations. The HRTR Core uses Oroboros Oxygraph-2k High Resolution Respirometry equipment. Applications of HRTR include: (1) diagnosis of acquired and genetic mitochondrial diseases, myopathies, and neuromuscular pathologies, (2) study of pathologies with reduced cellular respiration, (3) studies on cell function and cell death, (4) aging and senescence, (5) oxidative stress and antioxidant systems, (6) ischemia-reperfusion injury, (7) cancer research / pharmacological tests, (8) environmental stress, and (9) metabolic substrate balance.

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Last modified 02-07-2020

The Institute consists of 3 core laboratories—the Laboratory for Biochemistry, Molecular Phenotyping, and Microarray; the Laboratory for Statistical and Mathematical Genetics; and the Laboratory for Molecular Genetics, Bioinformatics, and High Throughput Genotyping. The Genomics Institute Faculty have been extremely active in large scale genetic studies and both single cohort and in multi-cohort (and multi-ethnic) consortium efforts that have identified many genetic loci for cardiometabolic traits. We have contributed to the design stages, to performance of biochemical phenotyping, to large scale genotyping, and to statistical genetic analysis. The many loci we and our collaborators have identified are being utilized by ourselves and others to generate multi-loci genetic risk scores as powerful tools to dissect disease etiology and to perform risk assessment in populations. Besides our involvement in many large scale genome wide association studies (GWAS), we are now actively involved in large scale whole genome sequencing studies (WGS) and Multi-Omics studies.

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Last modified 02-07-2020

The CTRC Bionutrition Core at Lundquist/Harbor-UCLA provides services to outpatient, overnight / extended stay and community based studies. A metabolic kitchen is dedicated to the preparation of nutrient controlled and regular meals. The Bionutrition Core has a metabolic assessment room with DXA, indirect calorimetry, BIA and anthropometric tools that integrates body composition, bone mineral density, and energy expenditure testing. The Bionutrition Core also provides state-of-the-art nutrition computing facilities with programs for nutrient analysis, semi-quantitative food frequency analysis, and design and specification of research diets. The Bionutrition Core personnel are experienced with REDCAP and can facilitate the collection and tracking of nutrition data for research studies. Training in nutrition research methodology is available to research fellows and other students.

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Last modified 02-07-2020

The Computed Tomographic (CT) Reading Core specializes in cardiac, vascular, and body composition imaging using advanced CT workstations, advanced quality control methods, systems storage, and analytical methods for multiple investigations. It provides unique services in atherosclerosis imaging including plaque quantification, perfusion imaging, and diagnostic services. In addition, the CT Reading Core offers collaborations in the area of renal, hepatic, vascular, and pulmonary imaging, as well as body composition and endothelial function assessment.

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Last modified 02-07-2020

The Endocrine and Metabolic Research Laboratory supports clinical and translational investigators in many aspects of research through expertise in analytical testing and development of new methods. Over forty different procedures are offered through the Endocrine and Metabolic Research Laboratory utilizing a variety of techniques including radioimmunoassay, fluoroimmunoassay, fluorometry and LC-MS/MS. Additionally, semen analyses and special andrological testing are available in the Andrology Laboratory.

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The pulmonary function testing (PFT) cardiopulmonary exercise testing (CPET) core lab facility supports investigators in pulmonary function and cardiopulmonary exercise testing studies. PFT consists of an array of non-invasive tests of abnormality of lung mechanics and pulmonary gas exchange used to detect the presence of, and quantify the severity of, a range of lung diseases. CPET provides a whole-body assessment of the integrative exercise responses involving the pulmonary, cardiovascular, hematopoietic, neuropsychological, and skeletal muscle systems, which are not adequately reflected through the measurement of individual organ system function. The CPET Core Lab will provide consultation, training, and services for clinical research using PFT and CPET. The Core Lab is also highly-experienced in multicenter clinical trials using PFT and CPET measurements.

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Last modified 02-07-2020

The Bioimaging and Immunotherapeutics Research Core provides four major resources to researchers: (1) the BD Calibur flow cytometer facility, which employs a dual-laser (argon and helium-neon), 4-color FACS Calibur flow cytometer and cell sorter to facilitate the molecular and physiological examination of individual cells and enable collection of living cells for further study; (2) the IVIS Lumina II system, a high-sensitivity, in vivo imaging technology platform that enables non-invasive visualization of both bioluminescent and fluorescent signals, tracking of cellular and therapeutic activities within a living organism in real time, and detection of signals from tissue, petri dishes, and microtiter plates; (3) a Luminex multiplex analyzer that utilizes xMAP microsphere technology and has the capability of performing up to 100 assays simultaneously in a single well of a microtiter plate; and (4) a Biotek Synergy 2 multi-mode microplate reader, a combination luminometer-fluorometer-absorbance detector capable of reading 96-well and larger plates that utilizes both filter-based and monochromatic-based light-detection technology.

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Last modified 02-07-2020

The Biomedical Mass Spectrometry Facility (BMSF) supports clinical research projects in clinical nutrition and metabolic research. The BMSF specializes in experimental design using stable isotopes and analytical methods (13C carbon tracing) for physiological or metabolic investigations. It provides unique services in isotopomer distribution analysis of metabolites (tracer-based metabolomics) using GC/MS (or LC-TOF). In addition, the BMSF offers collaborations in the area of metabolite profiling and modeling of metabolic systems with tracers. Services provided include: (1) sample preparation, (2) quantitative and qualitative analysis by GC/MS, IRMS and LC/MS, (3) consultation, (4) collaboration in metabolite profiling and tracer-based metabolomics, and (5) training on approved projects.

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The Guenther Molecular Biology Core supports investigators performing morphological, biochemical, cell and molecular biological studies both at cellular and subcellular levels. Consultation and training services are provided for localization of genes, RNA transcripts and proteins in complex tissues as well subcellular distribution in tissues. The laboratory houses five types of state-of the art equipment: an Agilent Bioanalyzer, two Applied Biosystem StepOne Plus Sequence Detection Systems, one Applied Biosystems 7900HT 384 well real-time quantitative PCR system, one Leica Confocal SP8 platform, and a Palm Microbeam. The Guenther Core also has a facility for immunohistochemistry studies.

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Last modified 02-07-2020

The Institute consists of 3 core laboratories—the Laboratory for Biochemistry, Molecular Phenotyping, and Microarray; the Laboratory for Statistical and Mathematical Genetics; and the Laboratory for Molecular Genetics, Bioinformatics, and High Throughput Genotyping. The Genomics Institute Faculty have been extremely active in large scale genetic studies and both single cohort and in multi-cohort (and multi-ethnic) consortium efforts that have identified many genetic loci for cardiometabolic traits. We have contributed to the design stages, to performance of biochemical phenotyping, to large scale genotyping, and to statistical genetic analysis. The many loci we and our collaborators have identified are being utilized by ourselves and others to generate multi-loci genetic risk scores as powerful tools to dissect disease etiology and to perform risk assessment in populations. Besides our involvement in many large scale genome wide association studies (GWAS), we are now actively involved in large scale whole genome sequencing studies (WGS) and Multi-Omics studies.

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Last modified 02-07-2020

Expertise and equipment for the design, implementation, and analysis of behavioral experiments. We work on a broad range of projects in both rats and mice, from behavioral phenotyping of novel transgenic mouse lines to developing rodent models of human clinical disorders.

Website:

http://btc.psych.ucla.edu/

Last modified 02-07-2020

The overall goal of the Center for Aids Research (CFAR) Humanized Mouse Core is to provide the infrastructure, materials, animals, technical expertise, and support that will facilitate the use of humanized immunodeficient mice in studies examining the pathogenesis and treatment of HIV-induced disease and cancer. Services include: (1) procuring immunodeficient mice at a reduced cost, (2) providing specialized animal laboratories for the construction, housing, and maintenance of immunodeficient mice, (3) constructing humanized mice, (4) developing and optimizing new humanized mouse models, (5) providing technical expertise and consultation on humanized mouse models. The core also provides consultation in flow cytometry, immunohistochemistry, PCR, and other assays.

Website:

https://www.uclahealth.org/aidsinstitute/cfar/humanized-mouse-core

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The ICP-MS Core Facility provides analytical chemistry characterization services to determine the qualitative and quantitative composition with wide element coverage, up to 80 elements. Chemical analysis is completed at a part per trillion (ppt, pg/mL) scale with high sensitivity. Trained staff will provide core services including: (1) Consultation for effective decision-making based on your needs, (2) Sample preparation (e.g., acid digestion), (3) Conduct analyses, and (4) Data reports with background corrections. Applications for the Core services include (but not limited to): (a) Drug delivery (drug release kinetics, biodistribution in different organs), (b) Concentration measurements of metal-containing proteins, (c) Cellular uptake, (d) Impurities / composition / stoichiometric ratio / ion release kinetics, (e) Toxic elements analysis (As, Pb, Cd, Hg, etc.) in food and soil, and (d) Trace metal analysis in drinking water and wastewater.

Website:

http://www.cein.ucla.edu/new/p193.php?pageID=409

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The Mouse Physiology Core Laboratory facility is dedicated to the phenotypic assessment of mouse and rat models of disease using a wide variety of integrative approaches. Our aim is to provide a cost effective means for investigators to reveal important functional aspects of their mouse and rat models without having to individually invest in hiring skilled personnel and in the purchase of expensive instrumentation. We specialize in ultrasound (echo) & telemetric assessments, survival and non-survival surgeries, acute and chronic evaluations of cardiac and neuro-regulatory function as well as basic metabolic and exercise assessments. The lab is also capable of certification testing of new drugs or substances intended for human use by performing FDA approved (GLP certified 21 CFR part 58 including part 11 compliance) studies on rodent models.

Website:

http://www.physiology.ucla.edu/Labs/Mouselab.htm

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The Radiation Oncology Animal Core breeds and houses mice in a gnotobiotic environment. The core can supply various strains of mice to meet investigators’ needs (please see website). Mice from other facilities may be imported, after inspection of sentinel health reports, and housed in the extra-barrier area. Irradiators are available for both whole-body and partial body exposures including image-guided techniques. The core additionally maintains an ABSL2 facility.

Website:

https://www.uclahealth.org/radonc/radiation-oncology-animal-core

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The Zebrafish Core facilitates the use and quick access of common mutation, genetically-engineered transgenic zebrafish, and routine techniques of zebrafish manipulation by investigators. Services include: (1) providing space to house zebrafish and perform large-scale genetic screens, (2) providing embryos for experiments, (3) generation of transgenic zebrafish, and (4) cryostorage of zebrafish sperm and re-derivation of live fish. Training is available for select techniques and zebrafish husbandry.

Website:

https://www.mcdb.ucla.edu/Research/Zebrafish/

Last modified 02-07-2020

The Cellular Bioenergetics Core provides access to the XF24-3, a unique instrument that quantifies the activity of two major cellular energetic pathways, mitochondrial respiration and glycolysis, in cultured cells. The XF Technology: (1) measures O2 consumption rate, extracellular acidification rate, and CO2 production rate of living cells simultaneously in real time, (2) does not require the addition of dyes, labels, or reporters, (3) is capable of assaying cell lines, primary cells, isolated mitochondria, and small tissue pieces, (4) typically requires only 30,000 to 80,000 cells/well in 24-well plates, (5) measures adherent cells without requiring trypsinization, (6) does not affect cells and plates, which can then be used for an additional assay, (7) allows measurements to be repeated multiple times to measure kinetic responses, and (8) accommodates the addition of up to four test compounds (drugs or substrates) to each well, with measurements performed before and after the addition of each compound.

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Last modified 02-07-2020

The Center for Aids Research (CFAR) Virology Core Laboratory provides a wide array of services for both HIV/AIDS and other researchers. It offers testing services (p24 ELISA, real time and digital droplet PCR for the detection of HIV cDNA, HIV viral load), blood and blood cell products. In addition, the core maintains a fully equipped and stocked BSL2+ facility for scientists that do not have wet lab space, new investigators joining UCLA or investigators that do not have such lab space. The UCLA/CFAR Virology Core Laboratory also maintains real time PCR instrumentation and a QX200 Bio Rad Digital Droplet PCR System. Finally,the core is able to assist individual researchers with special projects related to, but not limited, HIV detection.

Website:

http://www.virology.med.ucla.edu/

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The core laboratory provides instrumentation and technical and professional assistance for performing laser-based flow cytometric analysis and cell sorting. The facility also offers training in basic flow cytometry principles and in the operation of analytic cytometers. A second course teaches nucleic acid analysis and measurement of proliferation by flow cytometry as well as hands-on instrument set-up and use of the specialized DNA analysis software.

Website:

http://cyto.mednet.ucla.edu/home.html

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The Immune Assessment Core (IAC) is a CLIA certified laboratory that provides comprehensive immunological testing services for basic, clinical, and translational studies. The IAC provides both standardized and customized multi-parameter flow cytometry, multiplexed immunoassays and cellular immune function assays to evaluate the innate and adaptive immune status of study subjects. Validated tests include comprehensive immunophenotyping panels, multiplexed cytokine/chemokine detection in body fluids or culture supernatants, several ELISA assays, allo- and virus-specific T cell activation, T and B cell proliferation to antigens and mitogens, NK cell cytotoxicity, and granulocyte/monocyte oxidative burst. The core also performs customized assay development to meet the investigators’ needs.

Website:

http://pathology.ucla.edu/iac

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The Immuno/BioSpot Core specializes in assays related to the detection, quantification, and qualification of biological spot assays and single-cell related analyses, including ELISPOT, colony counting, plaque assays, FluoroSpot, cell viability tests, apoptosis tests, in vivo/in vitro cytotoxicity measurements using cells labeled with fluorescent dyes, histochemistry stains, genotoxicity assays, multi-color intracellular/surface quantification, etc.

Website:

Immuno/BioSpot Core

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The ICP-MS Core Facility provides analytical chemistry characterization services to determine the qualitative and quantitative composition with wide element coverage, up to 80 elements. Chemical analysis is completed at a part per trillion (ppt, pg/mL) scale with high sensitivity. Trained staff will provide core services including: (1) Consultation for effective decision-making based on your needs, (2) Sample preparation (e.g., acid digestion), (3) Conduct analyses, and (4) Data reports with background corrections. Applications for the Core services include (but not limited to): (a) Drug delivery (drug release kinetics, biodistribution in different organs), (b) Concentration measurements of metal-containing proteins, (c) Cellular uptake, (d) Impurities / composition / stoichiometric ratio / ion release kinetics, (e) Toxic elements analysis (As, Pb, Cd, Hg, etc.) in food and soil, and (d) Trace metal analysis in drinking water and wastewater.

Website:

http://www.cein.ucla.edu/new/p193.php?pageID=409

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The IMTC services include (but are not limited to): (1) consultation and troubleshooting in techniques for efficient gene delivery, as well as cloning and functional protein expression in mammalian cells in vitro and in vivo, (2) consultation and to perform customized gene expression profiling, cytological phenotype profiling, and high-throughput molecular screening studies by utilizing high-throughput and highly automated robotic systems. The Integrated Molecular Technologies Core (IMTC) is therefore intended to serve as a comprehensive resource that will provide access to state-of-the-art molecular tools and thereby aims to facilitate the research activities of CURE: DDRCC investigators.

Website:

https://www.ucladdrcc.med.ucla.edu/cores/integrated-molecular-technologies-core-imtc-and-microbiome-sub-core

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The UCLA Mitochondrial and Metabolism Core provides the necessary technical and conceptual resources for studying mitochondrial function. Services include respirometry assays in intact cells, permeabilized cells, and isolated mitochondria using the Seahorse XF96 Extracellular Flux Analyzer. An Operetta High Content Imaging System is also available for measurements of mitochondrial mass, membrane potential and reactive oxygen species, among other applications. The Mitochondrial and Metabolism Core provides in-depth training and scheduled use of instruments or full service options for assay design, performance, and data analysis.

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The AI in Imaging and Neuroscience Core provides automated and semi-automated processing of medical images with a special focus on vascular diseases and cancer using advanced pattern recognition and machine learning algorithms. A web-platform that offers a cloud computing pipeline is provided for the application of artificial intelligence to medical images and physiological signals. The services are centered on the processing of magnetic resonance imaging (MRI), computed tomography (CT), digital subtraction angiography (DSA), Transcranial Doppler ultrasound (TCD), intracranial pressure waveforms (ICP), digital pathology slides and time lapsed live cell microscopy videos.

Website:

http://web.cs.ucla.edu/~fab/core.html

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The Computing Technologies Research Laboratory (CTRL) specializes in research and clinical data collection, management, and reporting solutions. They develop Internet-accessible, web-based database applications that enable and enhance clinical & basic research and education, in the biomedical community. Services include: (1) web application development, (2) mobile app programming, (3) database design and hosting, and (4) digital video production / telemedicine for conferencing and the classroom.

Website:

https://dgit.healthsciences.ucla.edu/pages/Software-Development

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The primary objective of DOMStat is to provide study design and statistical analysis collaboration to investigators in the Department of Medicine. DOMStat offers faculty and staff level statistical collaboration for grant proposals, data analysis, manuscript preparation, and other research activities requiring statistical input.

Website:

http://domstat.med.ucla.edu

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The Informatics Center for Neurogenetics and Neurogenomics (ICNN) provides CNN faculty experts in statistical genetics, gene expression analysis, and bioinformatics will oversee the activities of highly-trained staff members who will accomplish three goals: 1) provide expert consultation and analyses for neurogenetics and neurogenomics projects; 2) develop and maintain a shared computing resource that will include a computational cluster for computation-intensive analyses, web-servers and state-of-the-art software tools for a wide range of applications (including user-friendly versions of public databases, as well as workstations on which ICNN users will be trained to employ these tools; and 3) provide hands-on training in analysis and informatics to the users. ICNN is developing a whole-genome data analysis pipeline, aimed at filtering and prioritizing genetic variation identified in whole-genome sequencing studies for further study.

Website:

https://www.semel.ucla.edu/research/core/informatics-center-neurogenetics-and-neurogenomics-icnn

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The Semel Institute Biostatistics Core (SIStat) is a team of expert biostatisticians, methodologists, and research support staff. The mission of the core is to foster research productivity and quality by helping faculty design sound research projects, obtain independent funding, and correctly analyze data. Services include: (1) development of electronic data entry systems, (2) biostatistics services, (3) development of patient assessment software, and (4) development of administrative systems software. Support is offered for projects ranging from small pilot studies to large, long-running, multi-site center studies.

Website:

http://www.sistat.ucla.edu/

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The UCLA Microbiome Core performs services related to microbiome studies including human sample collection, fecal processing, 16S ribosomal RNA gene sequencing (including feces, tissues, intestinal lavage, rectal sponges, vaginal swabs), bioinformatics analysis of 16S rRNA data in relation to phenotypes/interventions, quantitative PCR of total bacterial load, fecal calprotectin measurement, and long-term storage of fecal aliquots as well as extracted microbial DNA. The Microbiome Core also performs shotgun metagenomics and metatranscriptomics on a custom basis.

Website:

https://www.uclahealth.org/gastro/jacobs-lab/core-services

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The UCLA Neurosciences Genomics Core (UNGC) is currently operating an Illumina BeadLab 1000 high throughput SNP genotyping system (iScan), a Sequenom MassArray Compact mass spec and and two Illumina HiSeq 2500 next generation sequencing instruments in the Gonda research facility on the UCLA campus. Services include: RNA/DNA sequencing (Non UCLA neuro labs please inquire about service availability). Custom snp genotyping using the high throughput Illumina iSelect assays and with the Sequenom iPlex assay. Genotyping using all currently available Illumina Infinium whole genome chips. Gene expression using Illumina human expression chips. Whole genome methylation using Illumina Meth-450 chips. Project costs can be highly dependent on scale, with significant discounts potentially applying to larger projects.? For project specific pricing please contact? Joe DeYoung, Facility Manager.

Website:

https://www.semel.ucla.edu/ungc

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Technology Center for Genomics and Bioinformatics (TCGB) is a CAP-accredited, fully automated, high-throughput genomic facility equipped with all major next generation sequencing and microarray platforms. The Core provides pre-experiment consultation and post-experiment support, including preparation of grant applications and publications, and strategic planning for additional research steps. The TCGB also provides educational training to faculty, staff, and students to raise awareness on new directions and major discoveries in the areas of genomics and bioinformatics. Services include: (1) next generation sequencing, (2) microarray analysis, and (3) quality control services for RNA, miRNA, and DNA samples including RNA/DNA extraction, cDNA synthesis for quantitative-PCR, and qRT- PCR.

Website:

http://pathology.ucla.edu/tcgb

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The facility offers DNA extractions from whole blood and other tissue types, and storage of extracted DNA. BSPC uses the Autopure LS™ nucleic acid purification instrument from Gentra Systems for extracting DNA. This instrument provides a highly automated process with very high quality DNA, samples are quantitated using OD 260/280. Once DNA has been obtained from the provided sample, it can be returned immediately or stored in -20?C freezer for future use. We'll also aliquot DNA at the desired concentrations. DNA can be stored indefinitely within BSPC. All samples are logged in our database and are bar-coded with our sample number and customer's identification code. BSPC will soon initiate RNA extraction.

Website:

https://www.semel.ucla.edu/research/core/biological-samples-processing-core---bspc

Last modified 02-07-2020

The UCLA Center for Computer Vision and Imaging Biomarkers (CVIB) Laboratory provides global clinical and research services, including: (1) standardized multi-center imaging protocol development and quality control, (2) image de-identification, transfer, banking, and distribution, (3) cutting-edge quantitative image feature extraction, analysis, and data management, and (4) full service clinical trial management with regulatory compliance. CVIB has an exclusive contract with MedQIA Imaging CRO (www.medqia.com) to provide FDA-compliant services for industry-funded clinical trials worldwide. CVIB also provides clinical quantitative imaging services within UCLA Healthcare and to overseas hospital departments.

Key attributes of the CVIB Core Laboratory are: (1) image quality, (2) accurate and reproducible quantitation, (3) rigorous science, (4) high throughput, (5) well-managed and on-time service, and (6) user friendly.

Website:

http://cvib.ucla.edu/

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The Center for Human Nutrition provides Dual Energy X-Ray Absorptiometry (DXA or DEXA) Body Composition Analysis. DXA body composition analysis is considered the "gold standard" for measurement of body fat including visceral fat estimation, and gives an accurate measure for assessing health. Specialized scans to assess Bone Mineral Density analysis are available, which can be utilized for assessing bone health and risk estimation of osteopenia and osteoporosis.

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Last modified 02-07-2020

The Exercise Physiology Research Laboratory (EPRL) promotes a prevention model of wellness, focused on forestalling disease progression through the enhancement of health and fitness practices. Recently, the lab was distinguished as part of the Exercise is Medicine® on-campus recognition program through the American College of Sports Medicine, together whose vision is to create a campus culture that embraces physical activity and exercise as a daily facet of life and views it as essential for long-term health and wellness. Health-fitness assessments include: (1) aerobic performance; (2) pulmonary function; (3) body composition; (4) muscle performance; (5) functional movement screen; (6) total energy expenditure; (7) sleep quality; (8) cardiovascular health risk & HRV; (9) posture; and (10) performance tracking.

Website:

https://exerciselab.med.ucla.edu/

Last modified 02-07-2020

The Immune Assessment Core (IAC) is a CLIA certified laboratory that provides comprehensive immunological testing services for basic, clinical, and translational studies. The IAC provides both standardized and customized multi-parameter flow cytometry, multiplexed immunoassays and cellular immune function assays to evaluate the innate and adaptive immune status of study subjects. Validated tests include comprehensive immunophenotyping panels, multiplexed cytokine/chemokine detection in body fluids or culture supernatants, several ELISA assays, allo- and virus-specific T cell activation, T and B cell proliferation to antigens and mitogens, NK cell cytotoxicity, and granulocyte/monocyte oxidative burst. The core also performs customized assay development to meet the investigators’ needs.

Website:

http://pathology.ucla.edu/iac

Last modified 02-07-2020

The Immunogenetics Center provides comprehensive testing for organ and tissue transplantation to physicians, patients, pharmaceutical companies, and other medical facilities. It is one of the leading laboratories in the world, providing state-of-the-art technology and methodology with a comprehensive quality assurance program to ensure accuracy in testing. Services include: (1) HLA-class I (A,B,C) and class II (DR, DQ, DP) gene polymorphism typing, (2) histocompatibility testing (HLA antibody testing, MICA and endothelial cell antibody testing and crossmatch testing), (3) KIR and MICA gene typing and (4) immune assessment.

Website:

http://pathology.ucla.edu/uic-hla

Last modified 02-07-2020

Two previous research laboratories, the Clinical and Translational Research Laboratory (CTRL) and the Clinical Immunology Research Laboratory (CIRL) have been restructured into the Pathology Research Portal (PRP). This new addition to the Center for Pathology Research Services functions as the biospecimen liaison between researchers and clinical testing, and provides coordination for sample receiving, accessioning, processing, short term and long term storage, dispatching to multiple core facilities for testing, and result retrieving. PRP also provides shipping and temporary storage services, and can also provide customized services to meet various research needs.

Website:

http://pathology.ucla.edu/ctrl

Last modified 02-07-2020

The Transcranial Magnetic Stimulation (TMS) Lab providesTMS treatment to patients with Major Depressive Disorder (MDD) and other conditions.

Website:

http://www.tmslosangeles.com/index.php

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The Translational Pathology Core Laboratory (TPCL) provides an array of pathology-related services in support of basic, translational, and clinical research. Expert consultative services are offered to investigators in pathology-related study design, tissue selection, microscopic interpretation, immunohistochemistry/in situ hybridization, laser capture microdissection, digital image analysis, and IRB-related tissue questions. Services include: (1) tissue procurement, storage, and provision, (2) histology-related services, (3) immunohistochemistry, (4) in situ hybridization, (5) digital imaging and image analysis, (6) laser capture microdissection, (7) pathology consultative services, and (8) advice on research protocols, safety issues, and IRB applications.

Website:

http://pathology.ucla.edu/tpcl

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The mission of the Advanced Light Microscopy and Spectroscopy Laboratory (ALMS) is to provide consultation, services, and support for the application of novel microscopic and spectroscopic methods and advanced image analysis techniques for the study of macromolecules, cellular dynamics, and nano-scale characterization of bio-materials. The facilities provide a collection of high-level, customized biological fluorescence microscopes and small-animal imaging devices that provide the ability to study biological processes with high spatial and temporal resolution in whole organisms and in living cells down to the single molecule detection level with nanometer accuracy. Services include: (1) wide-field fluorescence imaging microscopy (on a limited basis), (2) confocal one-photon and two-photon laser scanning microscopy (point scanning and spinning disk), (3) fluorescence correlation spectroscopy, (4) fluorescence resonance energy transfer, (5) microscopic and macroscopic fluorescence lifetime imaging with time-correlated-single-photon-counting and near-infrared detection, (6) stimulated emission depletion laser-scanning microscopy, (7) microscopic and macroscopic (small animal) spectral unmixing, and (8) laser capture microdissection.

Website:

https://alms.cnsi.ucla.edu/

Last modified 02-07-2020

The Ahmanson-Lovelace Brain Mapping Center is committed to facilitating the development and growth of the field of Brain Mapping, a sub-discipline of neuroscience which uses brain imaging to better understand the structure and function of the human brain in health and alterations associated with disease. It is comprised of resources located in the core facility, the Ahmanson-Lovelace Brain Mapping Center, as well as additional resources housed in the Reed Neurological Research Center and the Neuroscience Research Building. Services include: (1) imaging (MRI and PET), (2) NeuroModulation, (3) data analysis, (4) data center, and (5) the DIVE (Data Immersive Visualization Environment).

Website:

http://www.bmap.ucla.edu/services/

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The Biological Chemistry Imaging Facility (BCIF) provides resources for data acquisition and analysis for radioactive, fluorescent, and photographic samples as well as digital imaging and document production. BCIF provides round-the-clock access to a cluster of modern equipment such as Typhoons 9410 and 9400 Variable Mode Imagers, Storm 820 and 840 imagers, Laser Densitometer, Gel Documentation System, color printers, and high-resolution scanners. In addition, BCIF provides unlimited scientific data storage space on its secure storage arrays to all participating labs and access to a number of software packages for data analysis and digital data processing.

Website:

http://www.biolchem.ucla.edu/biological-chemistry-imaging-facility

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The Electron Imaging Center for Nanomachines (EICN) provides state-of-the-art electron microscopy instruments and assisted usage services. Highly experienced staff provides necessary training and can help users address complex electron imaging needs. EICN offers advanced electron imaging techniques for visualizing materials, nanomachines, and cellular structures at atomic or nanometer scales in 2D and 3D. In addition, our latest integrated iCorr technology enables a streamlined approach to correlative microscopy to perform both fluorescence light and electron microscopy for biological research. Services available: (1) instrument training, (2) self usage, (3) assisted usage, and (4) data processing.

Website:

http://www.eicn.ucla.edu/

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The Electron Microscopy Services Center houses a JEOL 100CX transmission electron microscope. A Reichert Ultracut ultramicrotome is also available for use by trained personnel. Services include: (1) fixation and embedding of specimens, thin sectioning, (2) use of the electron microscopes (with or without assistance), (3) gold labeling, (4) negative stain, and (5) light microscopy of plastic embedded materials. The facility offers advice on appropriate preparatory procedures and other technical matters. Training and assistance in the use of the electron microscope is also offered.

Website:

http://www.bri.ucla.edu/research/core-facilities

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The Microscopic Techniques Laboratory offers instructions in microscopic techniques and assistance in tissue specimen preparation for light microscopic observation. Histological procedures available include: (1) some immunocytochemistry staining, (2) special stains, (3) paraffin sectioning, (4) slide preparation for in situ hybridization, (5) cryostat sectioning, (6) plastic embedding and (6) sectioning. The laboratory also provides staining setups, a cryostat, microtomes, and a Nikon photomicroscope for use by trained personnel.

Website:

http://www.bri.ucla.edu/research/core-facilities

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The MSSR is a core facility open to all research labs on campus. The services provided include the use of high throughput screening (HTS) technology, a total of roughly 200,000 compounds in various libraries, siRNA sets of the druggable genome for mouse and human and a database of results from screens. Please use the links above to find out more about the MSSR. The MSSR is currently funded by the JCCC, the Department of Molecular and Medical Pharmacology, the Department of Microbiology, Immunology and Molecular Genetics, and the Dean’s office, David Geffen School of Medicine and the California NanoSystems Institute.

Website:

https://mssr.cnsi.ucla.edu/

Last modified 02-07-2020

The Nano and Pico Characterization (NPC) Laboratory provides Provides both state-of-the-art microscopic techniques to visualize surfaces, adsorbates, nanostructures and devices at the atomic and molecular scale as well as a unique opportunity for researchers to gain insight into local properties under a wide range of experimental conditions.

Website:

https://npc.cnsi.ucla.edu/

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Provides state-of-the-art small animal imaging. It functions both as a shared preclinical imaging resource for UCLA researchers and as a hub for emerging imaging research and technology development. The same technologies and services are also available to the larger research community including other academic institutes and industry groups through contract work. The Imaging Center, operating through its sales-and-service, offers microPET, microCT, bioluminescence and fluorescence imaging modalities and complementary in vitro/ex vivo services including cell-based assays, biodistribution, digital autoradiography and dosimetry. Companion PET tracer radiochemistry and radiolabeling services are available in-house and is supported by on-campus cyclotron facilities. Technical and analytical support are available throughout the study process: initial consultation, experimental design and optimization, imaging protocols and techniques, post-acquisition data analysis and interpretation. Training and staff assistance The Imaging Center is part of the Crump Institute for Molecular Imaging and is supported by the expertise of its faculty members, world leaders in various imaging sciences.

Website:

https://imaging.crump.ucla.edu/

Last modified 02-07-2020

The Translational Research Imaging Center (TRIC) at UCLA is a state-of-the- art, pre-clinical and human cadaver, diagnostic and interventional imaging center. With over 25 years of expertise, our team of physicians, scientists, fellows, technologists, and veterinary staff support pre-clinical studies and imaging procedures across the field of medicine. TRIC is dedicated to the development and testing of new medical devices, imaging technologies, drug therapies as well as novel treatments. Our dedicated staff include: Board-certified Interventional Radiologists, Board-certified Radiologists, MR Physicists, Veterinarians, Statisticians, and Experienced Research Assistants. The TRIC Lab imaging equipment and support systems include: Magnetic Resonance Imaging (MRI) – Siemens Magnetom 3T Prisma MRI whole-body system, X-Ray Angiography – Siemens Artis Zeego Angiogram Suite with robotic C arm with 3D rotational angiography and DynaCT capabilities, Computed Tomography (CT) – Siemens Somatom Definition 64 Dual Source scanner, X-Ray Angiography – Philips Allura Xper FD-10 Angiogram Suite with floor mounted C arm with 3D rotational angiography capabilities, iU22 Philips Ultrasound system for general imaging, PACS data management system, observation and recovery suites, multi-modality 3D-image post-processing, High-Definition video integration for Telepresence video conferencing.

Website:

https://www.uclahealth.org/radiology/pre-clinical-services

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The IMTC services include (but are not limited to): (1) consultation and troubleshooting in techniques for efficient gene delivery, as well as cloning and functional protein expression in mammalian cells in vitro and in vivo, (2) consultation and to perform customized gene expression profiling, cytological phenotype profiling, and high-throughput molecular screening studies by utilizing high-throughput and highly automated robotic systems. The Integrated Molecular Technologies Core (IMTC) is therefore intended to serve as a comprehensive resource that will provide access to state-of-the-art molecular tools and thereby aims to facilitate the research activities of CURE: DDRCC investigators.

Website:

https://www.ucladdrcc.med.ucla.edu/cores/integrated-molecular-technologies-core-imtc-and-microbiome-sub-core

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The Integrated Nanomaterials Laboratory (INML) is a state-of-the-art nanomaterials synthesis and characterization facility. INML addresses the critical technological needs of the future through material development as nanoscience is integrated with disciplines such as electronics, photonics, renewable energy, chemistry, biology, and physics. Services include: (1) epitaxial services and research expertise and (2) molecular beam epitaxy facilities.

Website:

https://inml.cnsi.ucla.edu/

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The Integrated Systems Nanofabrication Cleanroom (ISNC) is a shared-use clean-room that provides state-of-the-art equipment, facilities, and technical expertise for fabricating the most demanding nano-scale structures and devices. The ISNC was created to support all aspects of nanofabrication-related research and to advance research and learning in nano-science. Services include: (1) e-beam lithography, (2) optical lithography, (3) thin-film deposition, (4) dry etch, (5) thermal processing, (6) metrology, and (7) access to integrated bio-suites.

Website:

https://isnc.cnsi.ucla.edu/

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Provides 3-dimensional structure of small molecules in solid crystals via X-ray crystallography. Equipped for characterization of polycrystalline solid materials using powder and thin film diffraction techniques. The laboratory is a full service facility where staff deals with all aspects of service from crystal selection to publication of results. In-house training is a requirement if you wish to use the equipment yourself. Part of the UCLA Molecular Instrumentation Center, which is a campus wide facility open to the UCLA community. The facility also collaborates with personnel from other universities and industry both locally and internationally.

Website:

http://uccore.org/ucla-j-d-mccullough-crystallography-laboratory-mic/

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The UCLA Lipidomics Laboratory provides the highest quality of quantitative lipid measurements from biological samples including cells, tissue, serum, and media. The lab offers automated sample preparation and two distinct mass spectrometry platforms: 1) shotgun lipidomics providing measurement of 1100 complex lipid species and 2) a fatty acid and sterols analysis compatible with isotopic labeling. Our full-service lab provides data analysis (including computational modeling of lipid flux parameters) and consultation services.

Website:

http://www.uclalipidomics.net/

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Consultation and technical assistance available to researchers prior to, and following, experiments. Evaluation of sample via Dynamic Light Scattering. Automatic setup of 4000 crystallization conditions per hour using 500uL of sample. (Hanging or Sitting Drops) Capable of distinguishing between organic and inorganic crystals using a sophisticated UV/vis microscope. Optimization of crystallization conditions. Storage of plates in temperature controlled incubators at 4?, 15?, and 20?C. Optimization of crystallization conditions using a library of 1,500 commercial compounds. Production of stereolithography files from Pymol, 3D design of labware, 3D printing.

Website:

http://www.doe-mbi.ucla.edu/crystallization/

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The Magnetic Resonance Facility houses six nuclear magnetic resonance (NMR) spectrometers and one electron paramagnetic resonance (EPR) spectrometer.

Website:

http://www.mic.ucla.edu/magnetic-resonance-spectroscopy

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The Mass Spectrometry Laboratory provides a wide range of sample characterization techniques for researchers. Mass spectrometry services include: (1) a variety of ionization methods including electron, chemical, matrix assisted laser desorption, direct analysis in real time, electrospray, and atmospheric pressure chemical ionization, (2) a variety of analyzers including time of flight, quadrupole, ion trap, Orbitrap, ion cyclotron resonance, and several tandem combinations of mass analyzers, and (3) microflow and nanoflow liquid chromatography. For proteomics studies, equipment is available for running and analyzing 1-D and 2-D gels, transferring mini- and mid-size gels, gel imaging, spot cutting, in-gel digestion, and protein/peptide identification. In addition, the facility provides consultation for sample preparation, experimental design, gel staining, analysis of protein expression patterns, western and lectin blots, in-gel trypic digestion, data mining, and operation of the various instruments. This laboratory is a part of the UCLA Molecular Instrumentation Center.

Website:

http://www.mic.ucla.edu/mass-spectrometry

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The Materials Characterization Laboratory offers thermal, optical, microscopic, electrical, and magnetic characterization of materials and elemental analysis of surfaces via a wide range of instruments including: (1) light scattering spectrometers, (2) several spectrophotometers, (3) scanning probe microscopes (AFM/STM), (4) a SQUID magnetometer, (5) a scanning electron microscope, and (6) an X-Ray photoelectron spectrometer. This laboratory is a part of the UCLA Molecular Instrumentation Center.

Website:

http://www.mic.ucla.edu/materials-characterization

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The UCLA Metabolomics Center enables investigators to study metabolism with a particular focus on central carbon metabolism (glycolysis, pentose phosphate pathway, TCA cycle, nucleos(t)ide synthesis, etc.). Metabolite analysis can be performed on a variety of specimens (e.g. cultured cells, culture medium, blood, urine, tissue). Generally, relative amounts of metabolites are measured, but absolute amounts can be determined as well. Tracing experiments using stable isotope-labeled metabolites (D, C-13, N-15) are routinely performed. For data analysis, relative amounts of metabolites as well as mass isotopologue distributions (if isotope tracing was performed) are calculated. Results are illustrated using bar graphs, heatmaps, principal component analysis, and mapping to Cytoscape maps.

Website:

http://systems.crump.ucla.edu/metabolomics_center/

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The MSSR is a core facility open to all research labs on campus. The services provided include the use of high throughput screening (HTS) technology, a total of roughly 200,000 compounds in various libraries, siRNA sets of the druggable genome for mouse and human and a database of results from screens. Please use the links above to find out more about the MSSR. The MSSR is currently funded by the JCCC, the Department of Molecular and Medical Pharmacology, the Department of Microbiology, Immunology and Molecular Genetics, and the Dean’s office, David Geffen School of Medicine and the California NanoSystems Institute.

Website:

https://mssr.cnsi.ucla.edu/

Last modified 02-07-2020

The NMR Core Facility provides advanced NMR spectrometers and related equipment for the study of macromolecular structure and dynamics in solution by experienced users. In this capacity the core facility primarily provides the research tools in which to perform detailed measurements on complex biological systems. Non-expert members of the DOE laboratory receive access to NMR spectroscopy for more routine applications. These applications include: screening wild-type and mutated protein samples to determine their structural integrity, making small molecule binding affinity measurements, and temperature and denaturant studies of protein stability.

Website:

https://www.doe-mbi.ucla.edu/nuclear-magnetic-resonance-nmr-core-technology-center/

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Provides consultation, training, and access to and technical assistance for metabolomics, proteomics (top-down and bottom-up) and targeted small molecule quantitation using mass spectrometery and contemporary chromatography.

Website:

https://massspec.chem.ucla.edu/

Last modified 02-07-2020

The Protein Expression Technology Center (PETC) was founded in 1994 to facilitate the expression and purification of proteins for structure/function studies. The PETC provides support in all aspects of protein expression from cloning through expression optimization and protein purification. The PETC is a UCLA-DOE Institute for Genomics and Proteomics facility but is open to all researchers.

Website:

http://www.doe-mbi.ucla.edu/expression/

Last modified 02-07-2020

The UCLA Proteome Research Center is a full-service technology center that offers a comprehensive suite of proteomic services to the UCLA community and other partnering institutions. By bringing together state-of-the-art instrumentation and high- level technical staff, the goal of the facility is to transform traditional experimental paradigms by providing investigators access to powerful analytical workflows for systems-level characterization of complex biological systems, biomarker discovery and validation, and protein complex characterization. Available services include: (1) complex protein mixture analysis using single and multidimensional fractionation strategies, (2) peptide quantitation using SILAC, iTRAQ / TMT, and label-free methodologies, (3) targeted and global PTM enrichment and characterization approaches, (4) identification of protein interaction networks using affinity-based isolation and proximity tagging techniques, and (5) targeted proteomic assay development using parallel reaction monitoring (PRM) for relative and absolute quantitation.

Website:

http://www.uclaproteomics.net/

Last modified 02-07-2020

The UCLA-DOE and Biochemistry Shared Instrumentation core facility provides the UCLA research community open access to sophisticated equipment for biochemical studies. These facilities house a wide array of detection systems useful for analysis, quantification, and identification of all types of macromolecules and their molecular interactions. It also carries equipment for cell culturing, harvesting, lysis, and high-speed centrifugation useful for biomolecule preparation.

Website:

http://biochem.cores.ucla.edu/general_info/

Last modified 02-07-2020

The X-ray Crystallography Core Technology Center in the Department of Engineering provides state-of-the-art resources to researchers, enabling the detailed 3-D analysis of biological macromolecules that play essential roles in human health. The facility operates as a full service core that offers access to sophisticated equipment and technologies as well as advice and technical assistance in sample preparation, data collection, processing, atomic refinement, and modeling. Services include: (1) aid in crystallization, (2) X-ray characterization of crystals, (3) X-ray data collection in-house and at the synchrotron, (4) processing and quality analysis of data, and (5) structure determination and display.

Website:

http://www.doe-mbi.ucla.edu/x-ray-crystallography-core-technology-center/

Last modified 02-07-2020

The Microelectronics Shop provides technical support for research, teaching, and administrative functions. Two IT Programmer/Analysts and one Electronics Engineer are on staff to provide electronic design and fabrication for custom electronics solutions to unique situations encountered in research projects. The staff has expertise in Macintosh, IBM clone computers, peripherals, printers, and network interfacing, including installation, setup, upgrades, demonstration, troubleshooting, repair, and modification services. The Microelectronics Shop also provides design, development, and installation of local area networks, specifying hubs, network cards, cable types, and software. Consultation is provided to determine appropriate hardware/software solutions.

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